Gottlieb M, Strober S, Kaplan H S
J Immunol. 1979 Jul;123(1):379-83.
BALB/c mice infused with 30 x 10(6) C57BL/Ka bone marrow (BM) cells 1 day after treatment with fractionated total lymphoid irradiation (TLI) (17 fractions of 200 rads each) became stable mixed chimeras without clinical graft-vs-host disease (GVHD). Mice given 18 fractions of 100, 50, or 25 rads each followed 1 day later by C57BL/Ka BM did not become chimeric, indicating that a critical cumulative radiation dose is required for this effect. Animals given TLI with lead shielding placed over the thymus also developed stable chimerism without GVHD. Thus susceptibility to tolerance induction and protection from GVHD after TLI and allogeneic BM transplantation is not due to alteration of the thymic microenvironment by fractionated irradiation. A delay of 7 or 21 days between completion of TLI and BM administration resulted in a high incidence of graft rejection. Sensitization to minor histocompatibility antigens of the BM donor strain by blood transfusion either before or during TLI resulted in marrow graft rejection in a high percentage of animals.
在接受分次全身淋巴照射（TLI）（每次200拉德，共17次）治疗1天后，给BALB/c小鼠输注30×10⁶个C57BL/Ka骨髓（BM）细胞，这些小鼠成为稳定的混合嵌合体，且无临床移植物抗宿主病（GVHD）。先接受每次100、50或25拉德，共18次的照射，1天后再给予C57BL/Ka BM的小鼠未形成嵌合体，这表明产生此效应需要临界累积辐射剂量。在胸腺上方放置铅屏蔽进行TLI照射的动物也形成了稳定的嵌合体且无GVHD。因此，TLI和同种异体BM移植后对耐受诱导的易感性以及对GVHD的保护并非由于分次照射改变了胸腺微环境。在TLI完成与BM输注之间延迟7天或21天会导致高比例的移植物排斥。在TLI之前或期间通过输血使小鼠对BM供体株的次要组织相容性抗原致敏，会导致高比例动物出现骨髓移植物排斥。
