Waer M, Ang K K, Van der Schueren E, Vandeputte M
J Immunol. 1984 Feb;132(2):991-6.
Different groups of C57BL/ka or BALB/c mice received a dose of 34 Gy or 42 Gy of fractionated total lymphoid irradiation (TLI) before bone marrow transplantation with 30 X 10(6) BALB/c or C57BL nucleated bone marrow cells, respectively. BALB/c mice that were not bred in specific pathogen-free conditions before TLI showed a high morbidity and mortality rate after 34 Gy of TLI and allogeneic bone marrow transplantation as compared with BALB/c or C57BL that were bred in pathogen-free conditions before irradiation. Many of the conventionally bred BALB/c mice had clinical and histologic signs of graft-vs-host disease after TLI and allogeneic bone marrow infusion. Although leucocytosis and lymphocytosis and the immunologic competence as measured with in vitro tests were equally depressed after 34 Gy TLI in BALB/c and C57BL mice, chimerism was nevertheless significantly easier to obtain in BALB/c mice. The incidence of chimerism after TLI could be enhanced in C57BL mice by increasing the total radiation dose from 34 to 42 Gy. This augmentation of chimerism was paralleled by the induction of more suppressor cells after 42 Gy of TLI in C57BL mice.
不同组的C57BL/ka或BALB/c小鼠在分别移植30×10⁶个BALB/c或C57BL有核骨髓细胞进行骨髓移植之前，接受了34 Gy或42 Gy的分次全身淋巴照射（TLI）。在TLI之前未在无特定病原体条件下饲养的BALB/c小鼠，与在照射前在无病原体条件下饲养的BALB/c或C57BL小鼠相比，在接受34 Gy的TLI和同种异体骨髓移植后显示出高发病率和死亡率。许多常规饲养的BALB/c小鼠在TLI和同种异体骨髓输注后出现移植物抗宿主病的临床和组织学迹象。尽管在BALB/c和C57BL小鼠中，34 Gy TLI后白细胞增多症、淋巴细胞增多症以及体外测试所测量的免疫能力同样受到抑制，但在BALB/c小鼠中嵌合体的获得却明显更容易。通过将总辐射剂量从34 Gy增加到42 Gy，C57BL小鼠TLI后嵌合体的发生率可以提高。在C57BL小鼠中，42 Gy的TLI后诱导出更多的抑制细胞与这种嵌合体的增加平行。
