通过传统的银-聚乙二醇纳米颗粒实现用于细胞成像的金属增强荧光。
Metal-enhanced fluorescence through conventional Ag-polyethylene glycol nanoparticles for cellular imaging.
作者信息
Chen Chih-Jung, Wu Chun-Yen, Wu Chi-Wei, Chang Ching-Wen, Huang Tsung-Tao, Shiao Ming-Hua, Lin Chu-Kuei, Chen Yu-Chun, Lin Yung-Sheng
机构信息
Department of Pathology and Laboratory Medicine, Taichung Veterans General Hospital Taichung 407219 Taiwan.
School of Medicine, Chung Shan Medical University Taichung 402306 Taiwan.
出版信息
RSC Adv. 2023 Sep 5;13(38):26545-26549. doi: 10.1039/d3ra02277c. eCollection 2023 Sep 4.
A novel application of conventional Ag nanoparticles (NPs) for metal-enhanced fluorescence (MEF) in cellular imaging is proposed. Different molecular weights of polyethylene glycol (PEG) were tested to determine a suitable spacer on Ag NPs for MEF, and NPs comprising Ag with PEG with a molecular weight of 6000 g (Ag-PEG6k), when present in fluorescein solution, were discovered to cause a 2-fold quantum yield enhancement. For fluorescence imaging of mesenchymal stem cells stained by Alexa Fluor 488, the enhancement factor increased with the Ag-PEG6k NP concentration but decreased with the Alexa Fluor 488 concentration. At 243 parts per billion Ag-PEG6k NPs and 625 parts per million Alexa Fluor 488, the enhancement factor reached its greatest value of over 4.
提出了传统银纳米颗粒（NPs）在细胞成像中用于金属增强荧光（MEF）的新应用。测试了不同分子量的聚乙二醇（PEG），以确定银纳米颗粒上适合用于MEF的间隔物，并且发现当存在于荧光素溶液中时，包含银与分子量为6000 g的PEG的纳米颗粒（Ag-PEG6k）会使量子产率提高2倍。对于用Alexa Fluor 488染色的间充质干细胞的荧光成像，增强因子随Ag-PEG6k纳米颗粒浓度的增加而增加，但随Alexa Fluor 488浓度的增加而降低。在十亿分之243的Ag-PEG6k纳米颗粒和百万分之625的Alexa Fluor 488时，增强因子达到其最大值，超过4。
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