Cai Zhenguo, Zeng Yibin, Liu Zhuohang, Zhu Ruizheng, Wang Wuqing
Department of Dermatology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, People's Republic of China.
Department of Dermatology, Changhai Hospital, Naval Medical University, Shanghai, 200433, People's Republic of China.
Clin Cosmet Investig Dermatol. 2023 Sep 1;16:2399-2408. doi: 10.2147/CCID.S423922. eCollection 2023.
To further investigate why curcumin (CUR) can attenuate psoriasis-like dermatitis of mice.
Sixteen mice were randomized into four groups. The control group used carrier cream, and the model and the CUR group were applied with topical 5% imiquimod in the naked mice skin once a day for 6 days (62.5 mg/day/mice). Meanwhile, the control and model mice were given the same dose of saline by oral means, while mice in the CUR groups received oral drug doses of 50 and 100 mg/kg once a day for 6 days, respectively. CUR could largely improve imiquimod-induced lesions of mice. By using the ELISA and qPCR, we found that the protein and mRNA levels of epidermal TNF-α and IL-6 were inhibited by CUR. The phosphorylation levels of STAT3 and its downstream associated protein levels (eg, Cyclin D1, Bcl-2 and Pim1) in skin tissues of different groups were also inhibited by CUR. Furthermore, the results of immunohistochemistry also showed the repressed effect of CUR for the expression of TNF-α, IL-6 and p-STAT3 in psoriasis-like lesions of mice.
CUR can effectively ameliorate the featured lesions of psoriasis mice, which may be closely associated with the involvement of IL-6/STAT3 signaling.
为进一步探究姜黄素（CUR）可减轻小鼠银屑病样皮炎的原因。
将16只小鼠随机分为四组。对照组使用载体乳膏，模型组和CUR组在裸鼠皮肤上每日涂抹一次5%咪喹莫特，共6天（62.5毫克/天/只）。同时，对照组和模型组小鼠经口给予相同剂量的生理盐水，而CUR组小鼠分别每日经口给予50和100毫克/千克的药物剂量，共6天。CUR可显著改善咪喹莫特诱导的小鼠皮损。通过酶联免疫吸附测定（ELISA）和定量聚合酶链反应（qPCR），我们发现表皮肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的蛋白质和信使核糖核酸（mRNA）水平受到CUR的抑制。不同组皮肤组织中信号转导和转录激活因子3（STAT3）的磷酸化水平及其下游相关蛋白水平（如细胞周期蛋白D1、Bcl-2和Pim1）也受到CUR的抑制。此外，免疫组织化学结果也显示CUR对小鼠银屑病样皮损中TNF-α、IL-6和磷酸化STAT3的表达具有抑制作用。
CUR可有效改善银屑病小鼠的特征性皮损，这可能与IL-6/STAT3信号通路的参与密切相关。
