Rubio M A, Engesser K H, Knackmuss H J
Arch Microbiol. 1986 Jul;145(2):116-22. doi: 10.1007/BF00446767.
Methylsalicylate-grown cells of Pseudomonas sp. WR401 cometabolized 3-, 4- and 5-substituted halosalicylates to the corresponding halocatechols. Further degradation was unproductive due to the presence of high levels of catechol 2,3-dioxygenase. This strain acquired the ability to utilize 3-chlorobenzoate following acquisition of genes from Pseudomonas sp. B13 which are necessary for the assimilation of chlorocatechols. This derivative (WR4011) was unable to use 4- or 5-chlorosalicylates. Derivatives able to use these compounds were obtained by plating WR4011 on 5-chlorosalicylate minimal medium; one such derivative was designated WR4016. The acquisition of this property was accompanied by concomitant loss of the methylsalicylate phenotype. During growth on 4- or 5-chlorosalicylate the typical enzymes of chlorocatechol assimilation were detected in cell free extracts, whereas catechol 2,3-dioxygenase activity was not induced. Repeated subcultivation of WR4016 in the presence of 3-chlorosalicylate produced variants (WR4016-1) which grew on all three isomers.
水杨酸甲酯培养的假单胞菌属WR401菌株将3 -、4 -和5 -取代的卤代水杨酸共代谢为相应的卤代儿茶酚。由于存在高水平的儿茶酚2,3 -双加氧酶，进一步的降解没有效果。该菌株在从假单胞菌属B13获得对氯儿茶酚同化所必需的基因后，获得了利用3 -氯苯甲酸的能力。这种衍生物（WR4011）不能利用4 -或5 -氯水杨酸。通过将WR4011接种在5 -氯水杨酸基本培养基上获得了能够利用这些化合物的衍生物；其中一种衍生物被命名为WR4016。获得这种特性的同时伴随着水杨酸甲酯表型的丧失。在以4 -或5 -氯水杨酸为生长底物时，在无细胞提取物中检测到了对氯儿茶酚同化的典型酶，而儿茶酚2,3 -双加氧酶活性未被诱导。在3 -氯水杨酸存在下对WR4016进行反复传代培养产生了能在所有三种异构体上生长的变体（WR4016 - 1）。
