假单胞菌中用于氯儿茶酚代谢的基因大量表达。
Abundant expression of Pseudomonas genes for chlorocatechol metabolism.
作者信息
Ngai K L, Ornston L N
机构信息
Department of Biology, Yale University, New Haven, Connecticut 06511.
出版信息
J Bacteriol. 1988 May;170(5):2412-3. doi: 10.1128/jb.170.5.2412-2413.1988.
Abstract
The respective specific activities of catechol 1,2-oxygenase II (catechol 1,2-dioxygenase; EC 1.13.11.1) and muconate cycloisomerase II (chloromuconate cycloisomerase; EC 5.5.1.7) in crude extracts of chlorobenzoate-grown Pseudomonas cells corresponded to about 16 and 11% of the soluble cell protein. High levels of protein synthesis appeared to compensate for a loss in catalytic activity that accompanied evolutionary acquisition of broad substrate specificity required for the enzymes to accommodate halogenated substrates.
摘要
在以氯苯甲酸为生长底物的假单胞菌细胞粗提物中，儿茶酚1,2 -加氧酶II（儿茶酚1,2 -双加氧酶；EC 1.13.11.1）和粘康酸环异构酶II（氯粘康酸环异构酶；EC 5.5.1.7）各自的比活性分别约为可溶性细胞蛋白的16%和11%。高水平的蛋白质合成似乎弥补了伴随酶进化获得适应卤代底物所需的广泛底物特异性而出现的催化活性损失。
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