Stevanović Oliver, Milanov Dubravka, Prošić Isidora, Gajdov Vladimir, Nedić Drago, Sladojević Željko, Radalj Andrea
1Veterinary Institute of the Republic of Srpska "Dr. Vaso Butozan", Branka Radičevića 18, Banja Luka 78000, Republic of Srpska, Bosnia and Herzegovina.
2Scientific Veterinary Institute "Novi Sad", Rumenački put 20, Novi Sad 21000, Serbia.
Acta Vet Hung. 2023 Sep 7;71(2):65-70. doi: 10.1556/004.2023.00893. Print 2023 Oct 17.
Nocardia cyriacigeorgica is a well-known agent of human nocardiosis and is considered an emerging pathogen, however, its identification to the species level is complex for many clinical laboratories. Available data on the clinical significance of N. cyriacigeorgica in veterinary medicine are sparse and mainly concern isolated reports of pyogranulomatous lesions in domestic animals. We report a case of severe bovine mastitis caused by N. cyriacigeorgica that did not respond to conventional antimicrobial therapy in a small holding in Bosnia and Herzegovina. After isolation of the pathogen, further identification by routine microbiological methods was not possible. Susceptibility to antimicrobials was tested using the disc diffusion method according to published recommendations. The sample was also tested by MALDI-ToF MS with inconclusive results. In addition, 16S rRNA sequence analysis, verified by multilocus sequence analysis (MLSA) using the gyrB, 16S rRNA, secA1, and hsp65 sequences, confirmed the species N. cyriacigeorgica. To our knowledge, this is the first report of isolation of N. cyriacigeorgica from a clinical case of bovine mastitis in a European dairy farm and the first MLSA method approach to distinguish a Nocardia spp. strain isolated from animals.
西里亚乔治诺卡菌是人类诺卡菌病的一种知名病原体，被视为一种新出现的病原体，然而，对于许多临床实验室来说，将其鉴定到种水平很复杂。关于西里亚乔治诺卡菌在兽医学中的临床意义的现有数据稀少，主要涉及家畜脓性肉芽肿病变的个别报道。我们报告了一例由西里亚乔治诺卡菌引起的严重牛乳腺炎病例，该病例发生在波斯尼亚和黑塞哥维那的一个小农场，对常规抗菌治疗无反应。病原体分离后，无法通过常规微生物学方法进行进一步鉴定。根据已发表的建议，使用纸片扩散法测试了对抗菌药物的敏感性。该样本也通过基质辅助激光解吸电离飞行时间质谱（MALDI-ToF MS）进行了检测，但结果不明确。此外，通过使用gyrB、16S rRNA、secA1和hsp65序列的多位点序列分析（MLSA）验证的16S rRNA序列分析，确认了西里亚乔治诺卡菌种。据我们所知，这是欧洲奶牛场临床牛乳腺炎病例中首次分离出西里亚乔治诺卡菌的报告，也是首次采用MLSA方法区分从动物分离出的诺卡菌属菌株。
