利用闪光分光光度法鉴定嗜盐菌中的第四种视紫红质样色素。

Flash spectrophotometric identification of a fourth rhodopsin-like pigment in Halobacterium halobium.

作者信息

Tomioka H, Takahashi T, Kamo N, Kobatake Y

出版信息

Biochem Biophys Res Commun. 1986 Sep 14;139(2):389-95. doi: 10.1016/s0006-291x(86)80003-1.

Abstract

A fourth retinal-containing pigment in Halobacterium halobium cell membrane was examined by flash spectrophotometry. The absorption maximum of this pigment was at about 480 nm. Flash light caused a photoreaction cycle with a half recovery time of about 300 ms at room temperature. The photoreaction cycle involved at least two photo-intermediates. The absorption maximum of the first one was at about 350 nm and that of the second was at around 530 nm. The spectral properties of this pigment and the content of the cells correlate with the sensitivity of photo-repellent response to the light around 480 nm. We suggest a name 'phoborhodopsin' for this new pigment.

摘要

通过闪光分光光度法对嗜盐菌细胞膜中第四种含视网膜的色素进行了检测。这种色素的最大吸收峰在约480纳米处。闪光在室温下引发了一个光反应循环，其半恢复时间约为300毫秒。该光反应循环涉及至少两种光中间体。第一种光中间体的最大吸收峰在约350纳米处，第二种在约530纳米左右。这种色素的光谱特性和细胞含量与避光反应对480纳米左右光的敏感性相关。我们建议将这种新色素命名为“避光视紫红质”。