Fife R S, Palmoski M J, Brandt K D
Arthritis Rheum. 1986 Oct;29(10):1256-62. doi: 10.1002/art.1780291011.
We have recently described a 550,000-dalton noncollagenous cartilage matrix glycoprotein (CMGP), with subunits of 130,000, which is present in hyaline cartilage and fibrocartilage. Biosynthetic studies indicated that CMGP was synthesized by short-term organ cultures of normal canine articular cartilage, representing approximately 9% of the total 3H-leucine incorporated into protein in 24-hour cultures. There was no incorporation of 35S-sulfate or 3H-mannose into CMGP under these conditions, but the protein did incorporate 32P-phosphate. The majority of the 3H-leucine-labeled CMGP was removed after 24 hours of chase with unlabeled leucine, and only a small amount remained at 72 hours, which suggests that there was rapid metabolism of the protein. CMGP was not detected in cartilage after addition of cycloheximide to the culture medium; this confirms its short half-life. Cultures of osteoarthritic cartilage obtained from dogs 8-10 weeks after anterior cruciate ligament transection revealed no difference in the metabolism of CMGP in this tissue compared with that found in cultures of normal articular cartilage.
我们最近描述了一种550,000道尔顿的非胶原蛋白软骨基质糖蛋白（CMGP），其亚基为130,000，存在于透明软骨和纤维软骨中。生物合成研究表明，CMGP由正常犬关节软骨的短期器官培养物合成，在24小时培养物中占掺入蛋白质的总3H-亮氨酸的约9%。在这些条件下，35S-硫酸盐或3H-甘露糖未掺入CMGP，但该蛋白质确实掺入了32P-磷酸盐。用未标记的亮氨酸追踪24小时后，大部分3H-亮氨酸标记的CMGP被去除，72小时时仅残留少量，这表明该蛋白质存在快速代谢。向培养基中添加环己酰亚胺后，在软骨中未检测到CMGP；这证实了其短半衰期。在前交叉韧带横断8 - 10周后从犬获取的骨关节炎软骨培养物显示，该组织中CMGP的代谢与正常关节软骨培养物中的代谢无差异。
