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PINE-TREE enables efficient enrichment of prime-edited hPSCs.

作者信息

Jia Mary S, Nelson Christopher E

机构信息

Department of Biomedical Engineering, University of Arkansas, Fayetteville, AR, USA.

Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR, USA.

出版信息

Mol Ther Nucleic Acids. 2023 Aug 29;33:908-909. doi: 10.1016/j.omtn.2023.08.021. eCollection 2023 Sep 12.

DOI:10.1016/j.omtn.2023.08.021
PMID:37680983
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10480562/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f980/10480562/51a6e6600850/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f980/10480562/51a6e6600850/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f980/10480562/51a6e6600850/gr1.jpg

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PINE-TREE enables efficient enrichment of prime-edited hPSCs.PINE-TREE能够高效富集经碱基编辑的人多能干细胞。
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本文引用的文献

1
PINE-TREE enables highly efficient genetic modification of human cell lines.PINE-TREE可实现对人类细胞系的高效基因改造。
Mol Ther Nucleic Acids. 2023 Jul 17;33:483-492. doi: 10.1016/j.omtn.2023.07.007. eCollection 2023 Sep 12.
2
Efficient prime editing in mouse brain, liver and heart with dual AAVs.双 AAV 高效在小鼠大脑、肝脏和心脏中进行的靶向碱基编辑。
Nat Biotechnol. 2024 Feb;42(2):253-264. doi: 10.1038/s41587-023-01758-z. Epub 2023 May 4.
3
Ex vivo prime editing of patient haematopoietic stem cells rescues sickle-cell disease phenotypes after engraftment in mice.
在体预编辑患者造血干细胞可在移植入小鼠后挽救镰状细胞病表型。
Nat Biomed Eng. 2023 May;7(5):616-628. doi: 10.1038/s41551-023-01026-0. Epub 2023 Apr 17.
4
Marker-free co-selection for successive rounds of prime editing in human cells.无标记共选择用于人类细胞中连续轮次的先导编辑。
Nat Commun. 2022 Oct 7;13(1):5909. doi: 10.1038/s41467-022-33669-z.
5
Designing and executing prime editing experiments in mammalian cells.在哺乳动物细胞中设计和执行先导编辑实验。
Nat Protoc. 2022 Nov;17(11):2431-2468. doi: 10.1038/s41596-022-00724-4. Epub 2022 Aug 8.
6
In vivo prime editing of a metabolic liver disease in mice.在体小鼠代谢性肝病的先导编辑。
Sci Transl Med. 2022 Mar 16;14(636):eabl9238. doi: 10.1126/scitranslmed.abl9238.
7
Mutation-specific reporter for optimization and enrichment of prime editing.用于优化和富集先导编辑的突变特异性报告基因。
Nat Commun. 2022 Mar 1;13(1):1028. doi: 10.1038/s41467-022-28656-3.
8
PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells.PEAR,一种灵活的荧光报告基因,可用于鉴定和富集成功进行的 Prime 编辑细胞。
Elife. 2022 Feb 23;11:e69504. doi: 10.7554/eLife.69504.
9
BIG-TREE: Base-Edited Isogenic hPSC Line Generation Using a Transient Reporter for Editing Enrichment.利用瞬时报告基因进行编辑富集的碱基编辑同基因 hPSC 系的生成
Stem Cell Reports. 2020 Feb 11;14(2):184-191. doi: 10.1016/j.stemcr.2019.12.013. Epub 2020 Jan 30.
10
A transient reporter for editing enrichment (TREE) in human cells.人类细胞中的编辑富集瞬态报告子(TREE)。
Nucleic Acids Res. 2019 Nov 4;47(19):e120. doi: 10.1093/nar/gkz713.