Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan.
Research Center for Materials Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan.
Chem Commun (Camb). 2023 Sep 26;59(77):11564-11567. doi: 10.1039/d2cc06189a.
We describe herein topological mRNA capture using branched oligodeoxynucleotides (ODNs) with multiple reactive functional groups. These fragmented ODNs efficiently formed topological complexes on template mRNA . In cell-based experiments targeting AcGFP mRNA, the bifurcated reactive ODNs showed a much larger gene silencing effect than the corresponding natural antisense ODN.
我们在此描述了使用具有多个反应性官能团的分支寡脱氧核苷酸 (ODN) 进行拓扑 mRNA 捕获。这些碎片化的 ODN 能够在模板 mRNA 上有效地形成拓扑复合物。在针对 AcGFP mRNA 的基于细胞的实验中，分叉的反应性 ODN 比相应的天然反义 ODN 显示出更大的基因沉默效果。
