Hashimoto S, Wisnieskie B J, Wong H
Biochim Biophys Acta. 1986 Oct 24;879(1):66-72. doi: 10.1016/0005-2760(86)90267-5.
The influence of chronic ethanol ingestion on hepatic acyl-CoA: cholesterol acyltransferase activity was investigated to determine the relationship between alcohol intake and cholesterol ester accumulation. Rats were given nutritionally complete liquid diets supplemented with 6.3% ethanol or an isocaloric equivalent of dextrin-maltose for 5 weeks. During this period, the hepatic acyl-CoA: cholesterol acyltransferase activity of ethanol-fed male rats remained constant, whereas the same activity in pair-fed controls as well as chow-fed rats exhibited a 30% decrease in activity. Unlike alcohol-fed male rats, the hepatic acyl-CoA: cholesterol acyltransferase activity of female rats decreased by approximately 30% by the fifth week of ethanol ingestion. Despite the fact that the gender of the animals led to disparate levels of acyl-CoA: cholesterol acyltransferase activity in response to ethanol ingestion, similar levels of cholesteryl ester accumulation were observed. The altered levels of acyl-CoA: cholesterol acyltransferase activity caused no significant change in the cholesterol concentration, cholesterol/phospholipid ratio, phospholipid fatty acid composition, or the membrane fluidity of the hepatic microsomes. We conclude that the altered hepatic acyl-CoA: cholesterol acyltransferase activity of ethanol-fed female rats cannot be directly responsible for ethanol-induced accumulation of cholesteryl esters.
研究了长期摄入乙醇对肝脏酰基辅酶A：胆固醇酰基转移酶活性的影响，以确定酒精摄入量与胆固醇酯积累之间的关系。给大鼠喂食营养完全的液体饮食，其中添加6.3%的乙醇或等热量的糊精 - 麦芽糖，持续5周。在此期间，喂食乙醇的雄性大鼠肝脏酰基辅酶A：胆固醇酰基转移酶活性保持恒定，而配对喂食的对照组以及正常饮食大鼠的相同活性则下降了30%。与喂食酒精的雄性大鼠不同，雌性大鼠在摄入乙醇的第五周时，肝脏酰基辅酶A：胆固醇酰基转移酶活性下降了约30%。尽管动物的性别导致对乙醇摄入的酰基辅酶A：胆固醇酰基转移酶活性水平不同，但观察到胆固醇酯积累水平相似。酰基辅酶A：胆固醇酰基转移酶活性水平的改变在胆固醇浓度、胆固醇/磷脂比率、磷脂脂肪酸组成或肝脏微粒体的膜流动性方面没有引起显著变化。我们得出结论，喂食乙醇的雌性大鼠肝脏酰基辅酶A：胆固醇酰基转移酶活性的改变不能直接导致乙醇诱导的胆固醇酯积累。
