Niccolai N, Bovalini L, Martelli P
Biophys Chem. 1986 Aug;24(3):217-20. doi: 10.1016/0301-4622(86)85027-x.
The furanochromones khellin and visnagin have been characterised by 1H and 13C mono- and bidimensional NMR spectroscopies. Their strong affinity with DNA was experimentally confirmed by the complete disappearance of the furano-chromones' NMR signals upon additions of DNA. An intermolecular interaction between furanochromones and the thymidyl moieties of DNA, stabilized by the formation of a hydrogen bond between the thymidyl NH hydrogen and the C = O group of khellin or visnagin, is here proposed. This is suggested by the strong donor-acceptor behavior of these two molecular moieties, as pointed out by a selective 1H-13C Overhauser effect study of the khellin-thymidine model system.
呋喃色酮凯林和紫花前胡内酯已通过一维和二维¹H及¹³C核磁共振光谱进行了表征。通过添加DNA后呋喃色酮的核磁共振信号完全消失，实验证实了它们与DNA具有很强的亲和力。本文提出呋喃色酮与DNA的胸苷部分之间存在分子间相互作用，这种相互作用通过胸苷NH氢与凯林或紫花前胡内酯的C=O基团之间形成氢键而得以稳定。如凯林 - 胸苷模型系统的选择性¹H-¹³C奥弗豪泽效应研究所示，这两个分子部分具有很强的供体 - 受体行为，表明了上述相互作用的存在。
