Lei Yujuan, Ma Zhansheng, Wang Ping, Qin Xuchen, Guan Xueqiang, Zhang Zhenwen
College of Food Science & Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China.
College of Food Engineering, Shihezi University, Shihezi 832000, China.
Foods. 2023 Aug 30;12(17):3258. doi: 10.3390/foods12173258.
The metabolic pathway of 3-alkyl-2-methoxypyrazines (MPs) in grape remains largely unclear except for the final step. In this study, the 2,5-dicarbonyl-3-isobutyl-piperazine (DCIP), which is proposed as the key intermediate of 3-isobutyl-2-methoxypyrazine (IBMP) biosynthesis, was incorporated into Cabernet Sauvignon clusters in using a soaking method. The IBMP concentration of grape and the expression patterns of VvOMTs in berry skin were monitored over two consecutive years. The results showed that the IBMP concentration of grape treated with DCIP was significantly increased at maturity in both years. The relative expression levels of and in berry skin were positively correlated with the IBMP accumulation. After DCIP incorporation, the relative expression level of and particularly that of were obviously up-regulated and closely mirrored the IBMP accumulation pattern in two consecutive years. Therefore, we speculate that DCIP may be a key intermediate involved in the biosynthesis of IBMP and plays an important role in regulating IBMP accumulation.
除了最后一步，葡萄中3-烷基-2-甲氧基吡嗪（MPs）的代谢途径在很大程度上仍不清楚。在本研究中，被认为是3-异丁基-2-甲氧基吡嗪（IBMP）生物合成关键中间体的2,5-二羰基-3-异丁基哌嗪（DCIP），通过浸泡法被引入赤霞珠葡萄串中。连续两年监测葡萄中IBMP的浓度以及浆果表皮中VvOMTs的表达模式。结果表明，两年中用DCIP处理的葡萄在成熟时IBMP浓度均显著增加。浆果表皮中 和 的相对表达水平与IBMP积累呈正相关。引入DCIP后， 和 特别是 的相对表达水平明显上调，并在连续两年中紧密反映了IBMP的积累模式。因此，我们推测DCIP可能是参与IBMP生物合成的关键中间体，并在调节IBMP积累中起重要作用。
