Nutrition and Cancer Laboratory, Department of Structural and Functional Biology, Institute of Biology, University of Campinas, Sao Paulo 13083-862, Brazil.
Int J Mol Sci. 2023 Aug 22;24(17):13026. doi: 10.3390/ijms241713026.
Cancer during pregnancy presents a delicate coexistence, imposing ethical and professional challenges on both the patient and medical team. In this study, we aimed to explore in a pre-clinical model the impact of tumour evolution in serum, placental and foetal metabolomics profiles during pregnancy in a time-course manner. Pregnant Wistar rats were distributed into two experimental groups: Control (C) and Walker-256 tumour-bearing (W). The rats were euthanised on three different gestational periods: at 12 days post-conception (dpc), at 16 dpc, and at 19 dpc. Serum, placenta and foetal metabolomic profiles were performed by H-NMR spectra following the analyses using Chenomx NMR Analysis Software V8.3. The tumour evolution was exponential, affecting the placental metabolomic profile during all the pregnancy stages. The placental tissue in tumour-bearing dams developed at a lower speed, decreasing the foetus's weight. Associated with the serum metabolomic changes related to tumour growth, the placental metabolomic alterations impacted many metabolic pathways related to energy provision, protein synthesis and signalling, which directly harmed the foetus's development. The development of the foetus is clearly affected by the damage induced by the tumour evolution, which alters the metabolic profile of both the serum and the placenta, impairing early embryonic development.
怀孕期间的癌症是一种微妙的共存状态，给患者和医疗团队带来了伦理和专业方面的挑战。在这项研究中，我们旨在通过临床前模型探索肿瘤在怀孕期间的血清、胎盘和胎儿代谢组学特征随时间的演变对其的影响。将怀孕的 Wistar 大鼠分为两组：对照组（C）和 Walker-256 肿瘤荷瘤组（W）。在三个不同的妊娠期（妊娠 12 天、16 天和 19 天）对大鼠进行安乐死。采用 H-NMR 谱分析血清、胎盘和胎儿代谢组学特征，使用 Chenomx NMR 分析软件 V8.3 进行分析。肿瘤的演变呈指数增长，影响整个妊娠期的胎盘代谢组学特征。肿瘤荷瘤母鼠的胎盘组织生长速度较慢，导致胎儿体重减轻。与与肿瘤生长相关的血清代谢变化相关，胎盘代谢组学的改变影响了许多与能量供应、蛋白质合成和信号转导相关的代谢途径，直接损害了胎儿的发育。肿瘤演变导致的损伤明显影响了胎儿的发育，改变了血清和胎盘的代谢特征，损害了早期胚胎发育。
