Department of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Halifax, NS B2N 5E3, Canada.
USDA, ARS, Grain Legume Genetics and Physiology Research Unit, Prosser, WA 99350, USA.
Int J Mol Sci. 2023 Aug 28;24(17):13311. doi: 10.3390/ijms241713311.
1021 bacteria secretes a considerable amount of flavins (FLs) and can form a nitrogen-fixing symbiosis with legumes. This strain is also associated with non-legume plants. However, its role in plant growth promotion (PGP) of non-legumes is not well understood. The present study evaluated the growth and development of lettuce () and kale ( var. acephala) plants inoculated with 1021 (FL) and its mutant 1021Δ, with a limited ability to secrete FLs (FL). The results from this study indicated that inoculation with 1021 significantly ( < 0.05) increased the lengths and surface areas of the roots and hypocotyls of the seedlings compared to 1021Δ. The kale and lettuce seedlings recorded 19% and 14% increases in total root length, respectively, following inoculation with 1021 compared to 1021Δ. A greenhouse study showed that plant growth, photosynthetic rate, and yield were improved by 1021 inoculation. Moreover, chlorophylls and , and total carotenoids were more significantly ( < 0.05) increased in kale plants associated with 1021 than non-inoculated plants. In kale, total phenolics and flavonoids were significantly ( < 0.05) increased by 6% and 23%, respectively, and in lettuce, the increments were 102% and 57%, respectively, following 1021 inoculation. Overall, bacterial-derived FLs enhanced kale and lettuce plant growth, physiological indices, and yield. Future investigation will use proteomic approaches combined with plant physiological responses to better understand host-plant responses to bacteria-derived FLs.
1021 细菌分泌大量黄素 (FL)，并能与豆科植物形成固氮共生关系。该菌株也与非豆科植物有关。然而，它在非豆科植物生长促进 (PGP) 中的作用还不是很清楚。本研究评估了接种 1021（FL）及其分泌 FL 能力有限的突变体 1021Δ对生菜（）和羽衣甘蓝（var. acephala）生长和发育的影响。研究结果表明，与 1021Δ相比，接种 1021 显著（<0.05）增加了幼苗的根和下胚轴的长度和表面积。与 1021Δ相比，接种 1021 后，羽衣甘蓝和生菜幼苗的总根长分别增加了 19%和 14%。温室研究表明，接种 1021 可提高植物生长、光合速率和产量。此外，与未接种植物相比，接种 1021 可显著（<0.05）增加羽衣甘蓝中叶绿素和类胡萝卜素以及总类胡萝卜素的含量。在羽衣甘蓝中，总酚类和类黄酮分别显著（<0.05）增加了 6%和 23%，而在生菜中，接种 1021 后分别增加了 102%和 57%。总的来说，细菌衍生的 FL 增强了羽衣甘蓝和生菜的植物生长、生理指标和产量。未来的研究将使用蛋白质组学方法结合植物生理反应来更好地理解宿主植物对细菌衍生的 FL 的反应。
