State Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
Int J Mol Sci. 2023 Aug 31;24(17):13509. doi: 10.3390/ijms241713509.
The growing occurrence of detrimental algal blooms resulting from industrial and agricultural activities emphasizes the urgency of implementing efficient removal strategies. In this study, we have successfully synthesized stable and biocompatible carbon dots (R-CDs) capable of generating reactive oxygen species (ROS) upon exposure to natural light irradiation. Phaeocystis globosa Scherffel (PGS) was selected as a representative model for conducting anti-algal experiments. Remarkably, in the presence of R-CDs, the complete eradication of harmful algae within a simulated light exposure period of 27 h was achieved. Furthermore, fluorescence lifetime imaging microscopy (FLIM) was first employed to study the physiological processes involved in the oxidative stress induced by PGS when subjected to ROS attack. The findings of this study demonstrate the potential of R-CDs as a highly promising anti-algal agent. This elucidation of the mechanism contributes to a comprehensive understanding of the efficacy and effectiveness of such agents in combating algal growth, further inspiring the development of other anti-algal agents.
由于工业和农业活动导致有害藻类大量繁殖，这凸显出实施高效去除策略的紧迫性。在本研究中，我们成功合成了稳定且具有生物相容性的碳点（R-CDs），它们在自然光照射下能够产生活性氧（ROS）。我们选择了聚球藻（PGS）作为代表性模型进行抗藻实验。值得注意的是，在 R-CDs 的存在下，在模拟光照暴露的 27 小时内，有害藻类被完全消灭。此外，我们首次利用荧光寿命成像显微镜（FLIM）研究了 PGS 在受到 ROS 攻击时诱导的氧化应激相关的生理过程。本研究的结果表明 R-CDs 作为一种很有前途的抗藻剂具有很大的潜力。该机制的阐明有助于全面了解此类试剂在抑制藻类生长方面的功效和有效性，进一步激发了其他抗藻剂的开发。
