Deletion of the Hinders Oligodendrocyte Progenitor Cell Development and Myelination in Zebrafish.

, an encoding ATP-dependent chromodomain helicase DNA-binding protein 7, has been identified as the causative gene involved in CHARGE syndrome (Coloboma of the eye, Heart defects, Atresia choanae, Retardation of growth and/or development, Genital abnormalities and Ear abnormalities). Although studies in rodent models have expanded our understanding of CHD7, its role in oligodendrocyte (OL) differentiation and myelination in zebrafish is still unclear. In this study, we generated a -knockout strain with CRISPR/Cas9 in zebrafish. We observed that knockout (KO) of intensely impeded the oligodendrocyte progenitor cells' (OPCs) migration and myelin formation due to massive expression of in oilg2 cells, which might provoke upregulation of the MAPK signal pathway. Thus, our study demonstrates that is critical to oligodendrocyte migration and myelination during early development in zebrafish and describes a mechanism potentially associated with CHARGE syndrome.