Grubliauskaite Monika, van der Perk M E Madeleine, Bos Annelies M E, Meijer Annelot J M, Gudleviciene Zivile, van den Heuvel-Eibrink Marry M, Rascon Jelena
Center for Pediatric Oncology and Hematology, Vilnius University Hospital Santaros Klinikos, Santariskiu Str. 4, LT-08406 Vilnius, Lithuania.
Life Sciences Center, Vilnius University, Sauletekio Ave. 7, LT-10257 Vilnius, Lithuania.
Cancers (Basel). 2023 Aug 22;15(17):4199. doi: 10.3390/cancers15174199.
Ovarian tissue cryopreservation and transplantation are the only available fertility techniques for prepubertal girls with cancer. Though autotransplantation carries a risk of reintroducing malignant cells, it can be avoided by identifying minimal infiltrative disease (MID) within ovarian tissue.
A broad search for peer-reviewed articles in the PubMed database was conducted in accordance with PRISMA guidelines up to March 2023. Search terms included 'minimal residual disease', 'cryopreservation', 'ovarian', 'cancer' and synonyms.
Out of 542 identified records, 17 were included. Ovarian tissues of at least 115 girls were evaluated and categorized as: hematological malignancies ( = 56; 48.7%), solid tumors ( = 42; 36.5%) and tumors of the central nervous system ( = 17; 14.8%). In ovarian tissue of 25 patients (21.7%), MID was detected using RT-qPCR, FISH or multicolor flow cytometry: 16 of them (64%) being ALL ( rearrangements with/without , , , fusion transcripts), 3 (12%) Ewing sarcoma ( fusion transcript, rearrangements), 3 (12%) CML ( fusion transcript, ) and 3 (12%) AML (leukemia-associated immunophenotypes, fusion transcript) patients.
While the majority of malignancies were found to have a low risk of containing malignant cells in ovarian tissue, further studies are needed to ensure safe implementation of future fertility restoration in clinical practice.
卵巢组织冷冻保存和移植是青春期前患癌女孩唯一可行的生育技术。尽管自体移植存在重新引入恶性细胞的风险，但通过识别卵巢组织内的微小浸润性疾病（MID）可以避免这种情况。
根据PRISMA指南，截至2023年3月在PubMed数据库中广泛检索同行评审文章。检索词包括“微小残留病”“冷冻保存”“卵巢”“癌症”及其同义词。
在542条识别记录中，纳入了17条。对至少115名女孩的卵巢组织进行了评估并分类为：血液系统恶性肿瘤（n = 56；48.7%）、实体瘤（n = 42；36.5%）和中枢神经系统肿瘤（n = 17；14.8%）。在25例患者（21.7%）的卵巢组织中，使用逆转录定量聚合酶链反应（RT-qPCR）、荧光原位杂交（FISH）或多色流式细胞术检测到了MID：其中16例（64%）为急性淋巴细胞白血病（ALL，有/无TEL-AML1、E2A-PBX1、BCR-ABL、MLL-AF4融合转录本），3例（12%）为尤因肉瘤（EWS-FLI1融合转录本，EWS rearrangements），3例（12%）为慢性粒细胞白血病（CML，BCR-ABL融合转录本，BCR rearrangements），3例（12%）为急性髓系白血病（AML，白血病相关免疫表型，MLL-AF4融合转录本）患者。
虽然发现大多数恶性肿瘤在卵巢组织中含有恶性细胞的风险较低，但仍需要进一步研究以确保未来在临床实践中安全实施生育力恢复。
Zotero 插件