Mejía-Méndez Jorge L, Lorenzo-Leal Ana C, Bach Horacio, López-Mena Edgar R, Navarro-López Diego E, Hernández Luis R, Juárez Zaida N, Sánchez-Arreola Eugenio
Laboratory of Phytochemistry Research, Chemical Biological Sciences Department, Universidad de las Américas Puebla, Ex Hacienda Sta. Catarina Mártir S/N, San Andrés Cholula 72810, Mexico.
Division of Infectious Diseases, Faculty of Medicine, University of British Columbia, Vancouver, BC V6H 3Z6, Canada.
Plants (Basel). 2023 Aug 31;12(17):3136. doi: 10.3390/plants12173136.
In this work, bulb extracts of were obtained by maceration with solvents of increasing polarity. The extracts were evaluated against a panel of pathogenic bacterial and fungal strains using the minimal inhibitory concentration (MIC) assay. The cytotoxicity of the extracts was tested against two cell lines (THP-1 and A549) using the MTT assay. The anti-inflammatory activity of the extracts was evaluated in THP-1 cells by measuring the secretion of pro-inflammatory (IL-6 and TNF-α) and anti-inflammatory (IL-10) cytokines by ELISA. The chemical composition of the extracts was recorded by FTIR spectroscopy, and their chemical profiles were evaluated using GC-MS. The results revealed that only hexane extract inhibited the growth of the clinical isolate of at 200 μg/mL. Against THP-1 cells, hexane and chloroform extracts were moderately cytotoxic, as they exhibited LC values of 90.16, and 46.42 μg/mL, respectively. Treatment with methanol extract was weakly cytotoxic at LC 443.12 μg/mL against the same cell line. Against the A549 cell line, hexane, chloroform, and methanol extracts were weakly cytotoxic because of their LC values: 294.77, 1472.37, and 843.12 μg/mL. The FTIR analysis suggested the presence of natural products were confirmed by carboxylic acids, ketones, hydroxyl groups, or esters. The GC-MS profile of extracts revealed the presence of phytosterols, tetracyclic triterpenes, multiple fatty acids, and sugars. This report confirms the antimicrobial, cytotoxic, and anti-inflammatory activities of .
在本研究中,通过用极性递增的溶剂浸渍获得了[植物名称]的鳞茎提取物。使用最低抑菌浓度(MIC)测定法对提取物针对一组致病细菌和真菌菌株进行了评估。使用MTT测定法针对两种细胞系(THP - 1和A549)测试了提取物的细胞毒性。通过酶联免疫吸附测定法(ELISA)测量促炎(IL - 6和TNF -α)和抗炎(IL - 10)细胞因子的分泌,在THP - 1细胞中评估了提取物的抗炎活性。通过傅里叶变换红外光谱(FTIR)记录提取物的化学成分,并使用气相色谱 - 质谱联用(GC - MS)评估其化学谱。结果表明,只有己烷提取物在200μg/mL时抑制了[细菌名称]临床分离株的生长。针对THP - 1细胞,己烷和氯仿提取物具有中等细胞毒性,因为它们的LC值分别为90.16和46.42μg/mL。甲醇提取物对同一细胞系的细胞毒性较弱,LC为443.12μg/mL。针对A549细胞系,己烷、氯仿和甲醇提取物具有较弱的细胞毒性,因为它们的LC值分别为294.77、1472.37和843.12μg/mL。FTIR分析表明存在天然产物,通过羧酸、酮、羟基或酯得以证实。提取物的GC - MS谱显示存在植物甾醇、四环三萜、多种脂肪酸和糖类。本报告证实了[植物名称]的抗菌、细胞毒性和抗炎活性。