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注射用富含 Decorin 的琼胶/黄原胶水凝胶包埋脂肪来源干细胞通过自噬信号增强软骨损伤的抗炎作用。

Injectable Decorin/Gellan Gum Hydrogel Encapsulating Adipose-Derived Stem Cells Enhances Anti-Inflammatory Effect in Cartilage Injury via Autophagy Signaling.

机构信息

Department of Orthopedic Surgery, Dongguan Hospital of Integrated Traditional Chinese and Western Medicine, Dongguan, China.

Department of Plastic Surgery, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.

出版信息

Cell Transplant. 2023 Jan-Dec;32:9636897231196493. doi: 10.1177/09636897231196493.

DOI:10.1177/09636897231196493
PMID:37688441
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10493051/
Abstract

Adipose-derived stem cells (ADSCs) are employed as a promising alternative in treating cartilage injury. Regulating the inflammatory "fingerprint" of ADSCs to improve their anti-inflammatory properties could enhance therapy efficiency. Herein, a novel injectable decorin/gellan gum hydrogel combined with ADSCs encapsulation for arthritis cartilage treatment is proposed. Decorin/gellan gum hydrogel was prepared according to the previous manufacturing protocol. The liquid-solid form transition of gellan gum hydrogel is perfectly suitable for intra-articular injection. Decorin-enriched matrix showing an immunomodulatory ability to enhance ADSCs anti-inflammatory phenotype under inflammation microenvironment by regulating autophagy signaling. This decorin/gellan gum/ADSCs hydrogel efficiently reverses interleukin-1β-induced cellular injury in chondrocytes. Through a mono-iodoacetate-induced arthritis mice model, the synergistic therapeutic effect of this ADSCs-loaded hydrogel, including inflammation attenuation and cartilage protection, is demonstrated. These results make the decorin/gellan gum hydrogel laden with ADSCs an ideal candidate for treating inflammatory joint disorders.

摘要

脂肪来源干细胞（ADSCs）被用作治疗软骨损伤的有前途的替代方法。调节 ADSCs 的炎症“指纹”以提高其抗炎特性可以提高治疗效率。本文提出了一种新型可注射的decorin/结冷胶水凝胶，结合 ADSCs 包封用于关节炎软骨治疗。根据先前的制造方案制备了decorin/结冷胶水凝胶。结冷胶水凝胶的液-固形式转变非常适合关节内注射。富含decorin 的基质表现出免疫调节能力，通过调节自噬信号来增强炎症微环境下 ADSCs 的抗炎表型。这种decorin/结冷胶/ADSCs 水凝胶可有效逆转白细胞介素-1β诱导的软骨细胞细胞损伤。通过单碘乙酸盐诱导的关节炎小鼠模型，证明了负载 ADSCs 的这种水凝胶的协同治疗效果，包括炎症减轻和软骨保护。这些结果使负载 ADSCs 的 decorin/结冷胶水凝胶成为治疗炎症性关节疾病的理想候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/9f2c97edae62/10.1177_09636897231196493-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/fb067260ad18/10.1177_09636897231196493-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/d38376c6c323/10.1177_09636897231196493-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/d283c139a081/10.1177_09636897231196493-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/553de43e3f5f/10.1177_09636897231196493-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/4aa47ed3d2c5/10.1177_09636897231196493-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/ec0e51084755/10.1177_09636897231196493-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/9f2c97edae62/10.1177_09636897231196493-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/fb067260ad18/10.1177_09636897231196493-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/d38376c6c323/10.1177_09636897231196493-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/d283c139a081/10.1177_09636897231196493-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/553de43e3f5f/10.1177_09636897231196493-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/4aa47ed3d2c5/10.1177_09636897231196493-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/ec0e51084755/10.1177_09636897231196493-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ff/10493051/9f2c97edae62/10.1177_09636897231196493-fig7.jpg

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Int J Biol Macromol. 2023 Apr 30;235:123525. doi: 10.1016/j.ijbiomac.2023.123525. Epub 2023 Feb 24.
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