Kruszewski F H, Chenicek K J, Digiovanni J
Cancer Lett. 1986 Sep;32(3):263-9. doi: 10.1016/0304-3835(86)90178-3.
Ornithine decarboxylase (ODC EC 4.1.1.17) induction in mouse epidermis after single or multiple topical applications of chrysarobin differed from that following topical application of 12-O-tetradecanoylphorbol-13-acetate (TPA). Following a single application of 220 nmol chrysarobin, ODC activity began to rise at 24 h reaching a peak at 56 h and returned to normal after 96 h. When 5 separate applications of 220 nmol chrysarobin were applied in multiple application protocols, an alteration in the ODC induction response was observed. With a once per week or twice per week application protocol, ODC was elevated in a multiphasic manner giving multiple peaks of activity after the last application. Interestingly, the magnitude of ODC induction was greater using the once per week, compared to the twice per week, application protocol. Preliminary results indicate that a once per week application protocol is more effective than a twice per week protocol for promoting the development of skin papillomas in SENCAR mice. Thus, the magnitude of the induced ODC response with chrysarobin, although low compared to TPA, correlated with tumor promoting activity.
单剂量或多剂量局部应用柯桠素后，小鼠表皮鸟氨酸脱羧酶（ODC，EC 4.1.1.17）的诱导情况与局部应用12 - O - 十四酰佛波醇 - 13 - 乙酸酯（TPA）后的情况不同。单次应用220 nmol柯桠素后，ODC活性在24小时开始上升，56小时达到峰值，96小时后恢复正常。在多剂量方案中，当分别5次应用220 nmol柯桠素时，观察到ODC诱导反应发生改变。采用每周一次或每周两次的应用方案时，ODC以多相方式升高，在最后一次应用后出现多个活性峰值。有趣的是，与每周两次的应用方案相比，每周一次的应用方案诱导的ODC幅度更大。初步结果表明，在促进SENCAR小鼠皮肤乳头状瘤的发生方面，每周一次的应用方案比每周两次的方案更有效。因此，尽管与TPA相比，柯桠素诱导的ODC反应幅度较低，但与肿瘤促进活性相关。
