鼠类和人类嗅组织染色技术。

Techniques in Staining of Rodent and Human Olfactory Tissue.

机构信息

Department of Otolaryngology-Head and Neck Surgery, The University of Tokyo, Tokyo, Japan.

Department of Otolaryngology-Head and Neck Surgery, Massachusetts Eye and Ear, Harvard Medical School, Boston, MA, USA.

出版信息

Methods Mol Biol. 2023;2710:195-207. doi: 10.1007/978-1-0716-3425-7_15.

DOI:10.1007/978-1-0716-3425-7_15

PMID:37688734

Abstract

Staining of olfactory tissue allows for evaluation of the organization of specific cell types within the specimen and allows for assessment in abnormalities of function that may be related to changes in normal tissue architecture. Histochemical staining and immunohistochemical (IHC) techniques are the most common methods for visualizing olfactory epithelium and olfactory bulbs. Here we describe the method of IHC for olfactory tissue utilizing both fluorescent and peroxidase-based methods of visualization.

摘要

嗅组织染色可评估标本中特定细胞类型的组织，并可评估可能与正常组织结构变化相关的功能异常。组织化学染色和免疫组织化学（IHC）技术是观察嗅上皮和嗅球最常用的方法。本文描述了利用荧光和过氧化物酶两种可视化方法进行嗅组织免疫组织化学染色的方法。