Young J T
Fundam Appl Toxicol. 1981 Jul-Aug;1(4):309-12. doi: 10.1016/s0272-0590(81)80037-1.
A method for preparation of the rat nasal cavity for histopathologic examination is presented, and the anatomic features of the sections obtained are described. The method results in four tissue sections which are selected because of their reproducibility based on landmarks on the dorsal aspect of the mouth and because of the anatomic features of each section which contribute to a thorough examination of the nasal cavity. The four areas examined include transverse sections taken at the following points: (1) immediately posterior to the upper incisor teeth, (2) at the incisive papilla, (3) at the second palatal ridge, and (4) at the level of the first upper molar teeth. The value of each section to the overall evaluation of the nasal cavity and adjacent structures is also discussed. The method described is applicable to both short-term and chronic toxicity studies, and allows for a thorough examination of the nasal cavity without destructive invasion at the time of necropsy.
本文介绍了一种用于制备大鼠鼻腔组织以进行组织病理学检查的方法，并描述了所获得切片的解剖学特征。该方法可得到四个组织切片，选择这四个切片是因为它们基于口腔背侧的标志具有可重复性，且每个切片的解剖学特征有助于对鼻腔进行全面检查。所检查的四个区域包括在以下位置获取的横切面：（1）上门齿后方紧邻处；（2）切牙乳头处；（3）第二腭嵴处；（4）第一上磨牙水平处。还讨论了每个切片对鼻腔及相邻结构整体评估的价值。所描述的方法适用于短期和慢性毒性研究，并且在尸检时无需进行破坏性侵入即可对鼻腔进行全面检查。
