Ottolenghi A
Cell Tissue Kinet. 1986 Jul;19(4):391-406. doi: 10.1111/j.1365-2184.1986.tb00737.x.
The correlation between the eosinophilic leucocyte population and the phospholipase B activity of rat tissues has been tested with isolated cell preparations from intestine, lung, blood, bone marrow and spleen containing eosinophils in varying proportions and with pure eosinophil fractions separated by centrifugation on discontinuous metrizoate and metrizamide gradients. A uniform value of activity per cell was found in all these tissues extending previous histochemical and biochemical evidence that the eosinophil is the carrier cell for the phospholipase B to all major sites of distribution. The enzyme marker approach has been used for estimating the normal eosinophil population of rat organs and show the distribution pattern of the eosinophils in peripheral organs in the wake of increased production and release from the marrow.
已使用来自肠道、肺、血液、骨髓和脾脏的分离细胞制剂对大鼠组织中嗜酸性白细胞群体与磷脂酶B活性之间的相关性进行了测试，这些细胞制剂含有不同比例的嗜酸性粒细胞，还使用了通过在不连续的泛影酸盐和甲泛葡胺梯度上离心分离得到的纯嗜酸性粒细胞组分。在所有这些组织中发现每个细胞的活性值均一，这扩展了先前的组织化学和生化证据，即嗜酸性粒细胞是磷脂酶B在所有主要分布部位的载体细胞。酶标记法已用于估计大鼠器官中的正常嗜酸性粒细胞群体，并显示了在骨髓中嗜酸性粒细胞产生和释放增加后，其在周围器官中的分布模式。
