Lingjaerde O, Monstad P
Cephalalgia. 1986 Sep;6(3):135-9. doi: 10.1046/j.1468-2982.1986.0603135.x.
A recently developed method for analysing 5-hydroxytryptamine (5-HT) efflux from platelets preloaded with a small amount of 14C-5-HT enables the assessment of the relative size of the granular and the cytoplasmatic pools of 5-HT within the platelets and of the rate of spontaneous efflux from these two compartments. This method, together with conventional assessment of the 5-HT uptake measures Km and Vmax, was applied in this study, comparing platelets from 14 patients with common migraine and 10 patients with classic migraine with platelets from 25 healthy controls. All patients were unmedicated and in an attack-free period. Neither the total patient group nor either of the two subgroups differed significantly from the control group on any measure of 5-HT uptake or efflux. However, two differences approached the conventional significance level: the relative size of the granular compartment (Compartment III) was larger for classic than for common migraine, and the efflux rate from Compartment III was shorter for classic migraine than for the healthy controls (P approximately 0.10 in both cases). Further studies are required to show whether these differences are real and, if so, whether they have any relevance for the pathogenesis of migraine attacks.
一种最近开发的用于分析从预先加载少量14C - 5 - 羟色胺（5 - HT）的血小板中5 - HT流出的方法，能够评估血小板内5 - HT颗粒池和细胞质池的相对大小以及这两个区室的自发流出速率。本研究应用了该方法以及对5 - HT摄取的常规评估来测定Km和Vmax，将14例普通偏头痛患者和10例典型偏头痛患者的血小板与25名健康对照者的血小板进行比较。所有患者均未用药且处于无发作期。在5 - HT摄取或流出的任何测量指标上，患者总体组以及两个亚组中的任何一个与对照组相比均无显著差异。然而，有两个差异接近传统显著性水平：典型偏头痛患者颗粒区室（区室III）的相对大小大于普通偏头痛患者，并且典型偏头痛患者区室III的流出速率短于健康对照者（两种情况P均约为0.10）。需要进一步研究以表明这些差异是否真实存在，如果存在，它们是否与偏头痛发作的发病机制有任何关联。
