IgE 抗体反应与广州管圆线虫感染宿主的脑病理损伤有关。

IgE antibody responses in cerebrospinal fluids relate to the brain pathologic injury of hosts with Angiostrongylus cantonensis infection.

机构信息

Department of Pediatrics, Tao-Yuan General Hospital, Taoyuan, Taiwan.

Department of Molecular Parasitology and Tropical Diseases, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; Center for International Tropical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.

出版信息

J Microbiol Immunol Infect. 2023 Dec;56(6):1261-1272. doi: 10.1016/j.jmii.2023.08.012. Epub 2023 Sep 4.

DOI:10.1016/j.jmii.2023.08.012

PMID:37689501

Abstract

BACKGROUND

The immunoglobulin E (IgE) response to Angiostrongylus cantonensis infection increases in the host. This study analyzed the IgG and IgE responses detected in different body fluids of A. cantonensis-infected mice.

METHODS

BALB/c (high susceptibility), CBA (medium), and C57BL/6 and C57BL/10 (resistance) strain mice were used in this study. The levels of IgM, IgG, and IgE in the serum and cerebrospinal fluid (CSF) from infected mice were compared. A. cantonensis-reactive antigens from BALB/c and C57BL/6 mice CSF were also analyzed.

RESULTS

Antibodies against fifth-stage larvae (L5) antigens increased in mice CSF, particularly IgE, relate to worm rejection and the susceptibility of different mouse strains. The increased IgE level in BALB/c mice CSF is lower than that from others, suggesting IgE response in brain is more important than that in serum. Anti-L5 and anti-excretory/secretory (ES) antigen IgE and IgG responses in CSF were analyzed. In addition, the antibody-dependent eosinophil-mediated cytotoxicity induced by anti-excretory/secretory (ES) antigen antibodies may be the reason of severe brain inflammation in infected BALB/c mice. IgE and IgG antibodies against a 105 kDa protein of L5 antigen was detected at week 3 post-infection in C57BL/6 mice and week 5 post-infection in BALB/c mice. We suggest that 105 kDa protein is related with the antibody response of A. cantonensis-infected mice.

CONCLUSION

We found that IgE antibodies in mice CSF against L5 antigens related to worm rejection in mice brains. This study may help to identify specific angiostrongyliasis markers that can be applied for clinical diagnosis and treatment in future.

摘要

背景

广州管圆线虫感染宿主后，免疫球蛋白 E（IgE）反应会增加。本研究分析了广州管圆线虫感染小鼠不同体液中 IgG 和 IgE 的反应。

方法

本研究使用 BALB/c（高易感性）、CBA（中易感性）、C57BL/6 和 C57BL/10（抗性）品系小鼠。比较感染小鼠血清和脑脊液（CSF）中的 IgM、IgG 和 IgE 水平。还分析了 BALB/c 和 C57BL/6 小鼠 CSF 中与广州管圆线虫反应的抗原。

结果

针对第五期幼虫（L5）抗原的抗体在小鼠 CSF 中增加，特别是 IgE，与蠕虫排斥和不同小鼠品系的易感性有关。BALB/c 小鼠 CSF 中 IgE 水平的升高低于其他小鼠，表明脑内 IgE 反应比血清中更重要。分析了 CSF 中针对 L5 和排泄/分泌（ES）抗原的 IgE 和 IgG 反应。此外，抗排泄/分泌（ES）抗原抗体引起的抗体依赖性嗜酸性粒细胞介导的细胞毒性可能是感染 BALB/c 小鼠严重脑炎症的原因。在感染后第 3 周和第 5 周，在 C57BL/6 小鼠和 BALB/c 小鼠中检测到针对 L5 抗原的 105 kDa 蛋白的 IgE 和 IgG 抗体。我们认为 105 kDa 蛋白与广州管圆线虫感染小鼠的抗体反应有关。