State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China.
School of Food and Health, Beijing Technology & Business University (BTBU), Beijing 100048, PR China.
Food Res Int. 2023 Oct;172:113108. doi: 10.1016/j.foodres.2023.113108. Epub 2023 Jun 10.
In this study, the impact of exopolysaccharides (EPS)-positive strain Weissella cibaria (W. cibaria) fermented sourdough on the quality of whole wheat fresh noodles (WWNs) and its improvement mechanisms were studied. The optimal fermentation conditions were found to be 30% sucrose content, fermented at 25 °C for 12 h, which yielded the highest EPS, 28.06 g/kg, in the W. cibaria fermented sourdough with sucrose (DW+). During storage, the sourdough reduced polyphenol oxidase activities and delayed the browning rate of noodles. The DW+ increased the hardness by 11.98% from 2184.99 to 2446.83 g, and the adhesiveness increased by 19.60%, i.e., from 72.01 to 86.13 g∙s of the noodles. The EPS mitigated acidification of sourdough, prevented the disaggregation of glutenin macropolymers (GMP), and increased sourdough elastic modulus. In addition, scanning electron microscope and confocal laser scanning microscopy of noodles containing EPS sourdough also demonstrated the uniform distribution of gluten proteins. The starch granules were also closely embedded in the gluten network. Thus, the present work indicated that the EPS produced sourdough delayed browning and improved the WWNs texture, indicating its potential to enhance the quality of whole grain noodles.
在这项研究中,研究了产胞外多糖(EPS)阳性魏斯氏菌 Weissella cibaria(W. cibaria)发酵酸面团对全麦鲜面条(WWNs)品质的影响及其改善机制。发现最佳发酵条件为 30%蔗糖含量,25°C 发酵 12 h,可使 W. cibaria 发酵的含蔗糖酸面团中 EPS 产量最高,为 28.06 g/kg。在贮藏过程中,酸面团降低了多酚氧化酶的活性,延缓了面条的褐变速度。DW+使面条的硬度从 2184.99 增加到 2446.83 g,增加了 11.98%,粘性从 72.01 增加到 86.13 g∙s,增加了 19.60%。EPS 缓解了酸面团的酸化,防止了醇溶谷蛋白大分子(GMP)的解聚,并增加了酸面团的弹性模量。此外,含有 EPS 酸面团的面条的扫描电子显微镜和共聚焦激光扫描显微镜也证明了面筋蛋白的均匀分布。淀粉颗粒也紧密嵌入面筋网络中。因此,本研究表明 EPS 产生的酸面团可延缓褐变,改善 WWNs 的质地,表明其具有提高全麦面条品质的潜力。