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基于 HS-SPME-GC-MS 联用电子鼻和电子舌的风味图谱分析不同等级九曲红梅茶的特征。

Characterization of different grades of Jiuqu hongmei tea based on flavor profiles using HS-SPME-GC-MS combined with E-nose and E-tongue.

机构信息

School of Life and Environmental Sciences, Shaoxing University, Shaoxing 312000, Zhejiang, China; National Engineering Research Center for Chinese CRW (Branch Center), Shaoxing University, 900 Chengnan Road, Shaoxing 312000, Zhejiang, China.

School of Life and Environmental Sciences, Shaoxing University, Shaoxing 312000, Zhejiang, China.

出版信息

Food Res Int. 2023 Oct;172:113198. doi: 10.1016/j.foodres.2023.113198. Epub 2023 Jul 5.

Abstract

In order to distinguish different grades of Jiuqu hongmei tea (black tea), four different grades of Jiuqu hongmei tea were used as materials in this study: Super Grade (SuG), First Grade (FG), Second Grade (SG), and Third Grade (TG). HS-SPME-GC-MS combined with electronic nose (E-nose) and electronic tongue (E-tongue) technology was used to detect and analyze tea samples. The results showed that 162 volatile substances were identified, mainly alcohols, followed by hydrocarbons, aldehydes, ketones and esters. Twenty-nine volatile compounds were found in all grades of tea samples. The results of heat map analysis showed that the relative contents of five volatile compounds in different grades of Jiuqu hongmei tea were positively correlated with the grades of Jiuqu hongmei tea. By orthogonal partial least squares discriminant analysis (OPLS-DA), 35 different compounds of SuG and FG, 30 different compounds of SG and TG, 34 different compounds of FG and SG were found. Overall, the results indicated that there were significant differences in volatile compounds among different grades of Jiuqu hongmei tea, and the use of HS-SPME-GC-MS combined with E-nose and E-tongue could provide a scientific reference method as an effective tool for detecting flavor characteristics of other types of black tea grades.

摘要

为了区分九曲红梅茶的不同等级,本研究选用特级(SuG)、一级(FG)、二级(SG)和三级(TG)四个不同等级的九曲红梅茶作为材料。采用顶空固相微萃取-气相色谱-质谱联用技术(HS-SPME-GC-MS)结合电子鼻(E-nose)和电子舌(E-tongue)技术对茶样进行检测和分析。结果表明,共鉴定出 162 种挥发性物质,主要为醇类,其次为烃类、醛类、酮类和酯类。在所有等级的茶样中均发现 29 种挥发性化合物。热图分析结果表明,不同等级九曲红梅茶中 5 种挥发性化合物的相对含量与九曲红梅茶的等级呈正相关。通过正交偏最小二乘判别分析(OPLS-DA),发现 SuG 和 FG 之间有 35 种不同的化合物,SG 和 TG 之间有 30 种不同的化合物,FG 和 SG 之间有 34 种不同的化合物。总体而言,结果表明不同等级的九曲红梅茶在挥发性化合物方面存在显著差异,HS-SPME-GC-MS 结合 E-nose 和 E-tongue 可提供一种科学的参考方法,作为检测其他类型红茶等级风味特征的有效工具。

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