School of Science, Anhui Agricultural University, 230036, Hefei, PR China.
School of Science, Anhui Agricultural University, 230036, Hefei, PR China.
Talanta. 2024 Jan 15;267:125169. doi: 10.1016/j.talanta.2023.125169. Epub 2023 Sep 8.
Lipid droplets (LDs) are important subcellular organelles that play a huge role in cell metabolism and growth. In this study, we synthesized two LDs fluorescent probes with benzothiadiazole (BTH) as electron acceptor and triphenylamine (TPA) as electron donor, which named as TPA-BTH1 and TPA-BTH2, respectively. Meanwhile, we introduced long alkyl chain to the probe as a shielding group and LDs targeting enhancement group. The results showed that the two probes were too sensitive to solvents' polarity because of the D-A structures possessed twisted intramolecular charge-transfer (TICT) effect. Furthermore, we prepared the two probes into nanoprobes by nanoprecipitation, which named as TPA-BTH1-20 and TPA-BTH2-20, respectively. The nanoprobes also had excellent fluorescence emission abilities and biocompatibility, as well as high photostability and accurately target LDs ability, which could be successfully applied in cell fluorescence imaging experiments.
脂滴（LDs）是细胞代谢和生长中起重要作用的亚细胞细胞器。在这项研究中，我们合成了两个以苯并噻二唑（BTH）为电子受体和三苯胺（TPA）为电子供体的 LDs 荧光探针，分别命名为 TPA-BTH1 和 TPA-BTH2。同时，我们在探针中引入长烷基链作为屏蔽基团和 LDs 靶向增强基团。结果表明，由于 D-A 结构具有扭曲的分子内电荷转移（TICT）效应，这两个探针对溶剂的极性过于敏感。此外，我们通过纳米沉淀法将这两个探针制备成纳米探针，分别命名为 TPA-BTH1-20 和 TPA-BTH2-20。纳米探针也具有优异的荧光发射能力和生物相容性，以及高的光稳定性和准确靶向 LDs 的能力，可成功应用于细胞荧光成像实验。
