College of Water Sciences, Beijing Normal University, Beijing, 100875, China.
College of Water Sciences, Beijing Normal University, Beijing, 100875, China; School of Statistics, Beijing Normal University, 100875, Beijing, China.
Environ Pollut. 2023 Nov 15;337:122529. doi: 10.1016/j.envpol.2023.122529. Epub 2023 Sep 8.
Microbial community, as the decomposers of constructed wetland (CW), plays crucial role in biodegradation and biotransformation of pollutants, nutrient cycling and the maintenance of ecosystem balance. In this study, 9 water samples, 6 sediment samples, and 8 plant samples were collected in Annan CW, which has the functions of water treatment and wetland culture park. The characteristics of microbial community structure in different media were illustrated by using of high-throughput sequencing-based metagenomics approach and statistical analysis. Meanwhile, this study identified and classified human pathogens in CW to avoid potential risks to human health. The results showed that dominant bacteria phyla in CW include Proteobacteria, Bacteroides, Actinobacteria, Firmicutes and Verrucomicrobia. The distribution of microorganisms in three media is different, but not significant. And the pH and DO profoundly affected microbe abundance, followed by water temperature. The microbial diversity in sediments is the highest, which is similar with the detection of human pathogens in sediments. Moreover, compared with Calamus, Lythrum salicaria and Reed, Scirpus tabernaemontani has fewer pathogenic microorganisms. The distribution of microorganisms in the CW is complex, and a variety of human pathogens are detected, which is more prone to create potential risks to human health and should receive additional attention.
微生物群落作为人工湿地（CW）的分解者，在污染物的生物降解和转化、营养物质循环以及生态系统平衡的维持中起着至关重要的作用。本研究在具有水处理和湿地文化公园功能的安南 CW 中采集了 9 个水样、6 个沉积物样本和 8 个植物样本。采用高通量测序宏基因组学方法和统计分析，阐述了不同介质中微生物群落结构的特征。同时，本研究鉴定并分类了 CW 中的人类病原体，以避免对人类健康的潜在风险。结果表明，CW 中的优势细菌门包括 Proteobacteria、Bacteroides、Actinobacteria、Firmicutes 和 Verrucomicrobia。三种介质中微生物的分布不同，但不显著。pH 值和 DO 对微生物丰度的影响较大，其次是水温。沉积物中的微生物多样性最高，与沉积物中人类病原体的检测结果相似。此外，与香蒲、千屈菜和芦苇相比，藨草的致病微生物较少。CW 中的微生物分布复杂，检测到多种人类病原体，更容易对人类健康造成潜在风险，应引起更多关注。
