Liu Yuh-Hwa, Lin Yin-Shiou, Sie Yi-Yan, Wang Ching-Chiung, Chang Chi-I, Hou Wen-Chi
Department of General Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, 110, Taiwan.
Division of Gastroenterology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, 111, Taiwan.
J Tradit Complement Med. 2023 May 30;13(5):479-488. doi: 10.1016/j.jtcme.2023.05.003. eCollection 2023 Sep.
In Taiwan, var. (VTT) is used in traditional medicine and as a local tea. VTT rich in resveratrol and resveratrol oligomers have been reported to exhibit anti-obesity and anti-hypertensive activities in animal models; however, no studies have investigated type 2 diabetes mellitus (T2DM) treatments. This study aimed to investigate the anti-T2DM effects of resveratrol tetramers isolated from the VTT in nicotinamide/streptozotocin (STZ)-induced Institute of Cancer Research (ICR) mice.
The oral glucose tolerance test (OGTT) was used to imitate postprandial blood glucose (BG) regulations in mice by pre-treatment with VTT extracts, resveratrol tetramers of vitisin A, vitisin B, and hopeaphenol 30 min before glucose loads. Vitisin B (50 mg/kg) was administered to treat T2DM-ICR mice once daily for 28 days to investigate its hypoglycemic activity.
Mice pre-treated with VTT-S-95EE, or vitisin B (100 mg/kg) 30-min before glucose loading showed significant reductions ( < 0.001) in the area under the curve at 120-min (BG-AUC) than those without pre-treatment with VTT-S-95 E E or vitisin B. Vitisin B-treated T2DM mice showed hypoglycemic activities via a reduction in plasma dipeptidyl peptidase (DPP)-IV activities to maintain insulin actions and differed significantly than those of untreated T2DM mice ( < 0.05), and also reduced BG-AUC and insulin-AUC in the OGTT.These results showed that VTT containing vitisin B would be beneficial for developing nutraceuticals and/or functional foods for glycemic control in patients with T2DM, which should be investigated further.
在台湾，葡萄变种（VTT）被用于传统医学并作为当地茶饮。据报道，富含白藜芦醇和白藜芦醇低聚物的VTT在动物模型中具有抗肥胖和抗高血压活性；然而，尚无研究调查其对2型糖尿病（T2DM）的治疗作用。本研究旨在探讨从VTT中分离出的白藜芦醇四聚体对烟酰胺/链脲佐菌素（STZ）诱导的癌症研究所（ICR）小鼠的抗T2DM作用。
口服葡萄糖耐量试验（OGTT）用于通过在葡萄糖负荷前30分钟用VTT提取物、葡萄素A、葡萄素B和蛇麻脂酚的白藜芦醇四聚体预处理来模拟小鼠餐后血糖（BG）调节。给予葡萄素B（50mg/kg）每日一次治疗T2DM-ICR小鼠，持续28天，以研究其降血糖活性。
在葡萄糖负荷前30分钟用VTT-S-95EE或葡萄素B（100mg/kg）预处理的小鼠，其120分钟时曲线下面积（BG-AUC）比未用VTT-S-95EE或葡萄素B预处理的小鼠显著降低（P<0.001）。葡萄素B治疗的T2DM小鼠通过降低血浆二肽基肽酶（DPP）-IV活性以维持胰岛素作用而表现出降血糖活性，与未治疗的T2DM小鼠相比有显著差异（P<0.05），并且在OGTT中也降低了BG-AUC和胰岛素-AUC。这些结果表明，含有葡萄素B的VTT将有利于开发用于T2DM患者血糖控制的营养保健品和/或功能性食品，对此应进一步研究。
