Makker S P, Aikawa M
Lab Invest. 1979 Jul;41(1):45-50.
A mesangial glomerulonephropathy, characterized by the deposition of rat IgG, IgM, and C3 in the glomerular mesangium, was produced in Wistar rats by a prolonged administration of mercuric chloride (HgCl2). The HgCl2 was dissolved in sterile distilled water (0.2 mg. per ml.), and a group of 15 male Wistar rats was given injections subcutaneously three times a week on alternate days at a dosage of 0.15 mg. per 100 gm. of body weight for 27 weeks. A control group of nine rats was given injections of distilled water only. Mesangial glomerulonephropathy developed in 12 of 15 rats injected with HgCl2 and was characterized by the following: (1) coarse granular and nodular deposition of rat IgG, IgM, and C3 in the mesangium of all glomeruli, (2) absence of staining for rat albumin, IgA, and fibrin, (3) presence of electron-dense deposits in the mesangium, (4) focal and segmental proliferation of the mesangial matrix, (5) interstitial inflammation, (6) tubular atrophy, and (7) deposition of periodic acid-Schiff-positive material in the medulla adjacent to the thin limbs of the loops of Henle. Glycosuria and a slight increase in proteinuria were observed transiently in some rats. The blood urea nitrogen levels were normal in all rats. Eluates from the kidneys with heavy mesangial deposits contained rat IgG. However, the eluted antibody failed to react with normal rat kidney tissue components. None of the above findings were present in the control rats. The study provides a model of a mesangial nephropathy that seems to be immunologically induced; however, the mechanism for the formation and deposition of the immune deposits containing rat IgG, IgM, and C3, and the nature of the antigen(s) have not been elucidated.
通过长期给予氯化汞（HgCl₂），在Wistar大鼠中诱发了一种系膜增生性肾小球肾炎，其特征是大鼠IgG、IgM和C3在肾小球系膜中沉积。将HgCl₂溶解于无菌蒸馏水中（每毫升0.2毫克），一组15只雄性Wistar大鼠每周皮下注射3次，隔日注射，剂量为每100克体重0.15毫克，持续27周。另一组9只大鼠作为对照组，仅注射蒸馏水。注射HgCl₂的15只大鼠中有12只发生了系膜增生性肾小球肾炎，其特征如下：（1）所有肾小球系膜中大鼠IgG、IgM和C3呈粗大颗粒状和结节状沉积；（2）大鼠白蛋白、IgA和纤维蛋白染色阴性；（3）系膜中有电子致密沉积物；（4）系膜基质局灶性和节段性增生；（5）间质炎症；（6）肾小管萎缩；（7）在靠近髓袢细段的髓质中有过碘酸希夫阳性物质沉积。部分大鼠短暂出现糖尿和蛋白尿略有增加。所有大鼠的血尿素氮水平均正常。系膜重度沉积的肾脏洗脱液中含有大鼠IgG。然而，洗脱的抗体未能与正常大鼠肾脏组织成分发生反应。上述所有表现均未在对照大鼠中出现。该研究提供了一种似乎由免疫诱导的系膜肾病模型；然而，含大鼠IgG、IgM和C3的免疫沉积物的形成和沉积机制以及抗原的性质尚未阐明。
