糖皮质激素在细胞外改变III型前胶原与I型前胶原的比例。
Glucocorticoids change the ratio of type III to type I procollagen extracellularly.
作者信息
Shull S, Cutroneo K R
出版信息
Coll Relat Res. 1986 Jul;6(3):295-300. doi: 10.1016/s0174-173x(86)80013-9.
Rat skin fibroblasts were treated with dexamethasone and labeled with radioactive proline for 2 hours. Intracellular and extracellular procollagens were immunoprecipitated with monospecific polyclonal antibodies. Both intracellular and extracellular types I and III procollagen were decreased coordinately by glucocorticoid treatment. The ratio of type III to type I procollagen was the same for control and dexamethasone-treated cell cultures. However, when rat skin fibroblasts were prelabeled with radioactive proline and chased in the presence of unlabeled proline the ratio of type III to type I extracellular procollagens remained constant in the glucocorticoid-treated cells and increased in control fibroblasts.
用 dexamethasone 处理大鼠皮肤成纤维细胞，并用放射性脯氨酸标记 2 小时。用单特异性多克隆抗体免疫沉淀细胞内和细胞外的前胶原。糖皮质激素处理使细胞内和细胞外的 I 型和 III 型前胶原均协同减少。对照和经 dexamethasone 处理的细胞培养物中 III 型与 I 型前胶原的比例相同。然而，当用放射性脯氨酸预标记大鼠皮肤成纤维细胞并在未标记脯氨酸存在的情况下进行追踪时，糖皮质激素处理的细胞中细胞外 III 型与 I 型前胶原的比例保持恒定，而对照成纤维细胞中的该比例增加。
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