National Laboratory of Solid State Microstructures, Collaborative Innovation Center of Advanced Microstructures, Chemistry and Biomedicine Innovation Center, College of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing 210093, People's Republic of China.
National Laboratory of Solid State Microstructures, Collaborative Innovation Center of Advanced Microstructures, School of Physics, Nanjing University, Nanjing 210093, People's Republic of China.
Nano Lett. 2023 Sep 27;23(18):8770-8778. doi: 10.1021/acs.nanolett.3c02889. Epub 2023 Sep 11.
Multicellular 3D tissue constructs (MTCs) are important in biomedical research due to their capacity to accurately mimic the structure and variation found in real tissues. This study presents a novel bio-orthogonal engineering strategy (BIEN), a transformative scaffold-free approach, to create advanced MTCs. BIEN harnesses the cellular biosynthetic machinery to incorporate bio-orthogonal azide reporters into cell surface glycoconjugates, followed by a click reaction with multiarm PEG, resulting in rapid assembly of MTCs. The implementation of this cutting-edge strategy culminates in the formation of uniform, heterogeneous spheroids, characterized by a high degree of intercellular junction and pluripotency. Remarkably, MTCs simulate tumor features, ensure cell heterogeneity, and significantly improve the subcutaneous xenograft model after transplantation, thereby bolstering both and research models. In conclusion, the utilization of the bio-orthogonal engineering strategy as a scaffold-free method to generate superior MTCs holds promising potential for driving advancements in cancer research.
多细胞 3D 组织构建体（MTCs）在生物医学研究中非常重要，因为它们能够准确模拟真实组织中的结构和变化。本研究提出了一种新颖的生物正交工程策略（BIEN），这是一种变革性的无支架方法，可用于构建先进的 MTCs。BIEN 利用细胞生物合成机制将生物正交叠氮报告物掺入细胞表面糖缀合物中，然后与多臂 PEG 进行点击反应，从而快速组装 MTCs。这种尖端策略的实施最终形成了均匀的、异质的球体，具有高度的细胞间连接和多能性。值得注意的是，MTCs 模拟了肿瘤特征，确保了细胞异质性，并在移植后显著改善了皮下异种移植模型，从而增强了 和 研究模型。总之，生物正交工程策略作为一种无支架方法来生成优越的 MTCs 具有很大的潜力，可以推动癌症研究的进展。
