本地群体感应淬灭细菌代尔夫特菌JL5对膜生物反应器中生物污染的缓解作用及微生物群落动态
Biofouling mitigation and microbial community dynamics in the membrane bioreactor by the indigenous quorum quenching bacterium Delftia sp. JL5.
作者信息
Xu Fangfang, Liao Jialong, Hu Jinchen, Feng Yunshi, Huang Yanyao, Feng Xingtong, Li Shuangfei
机构信息
Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China; Forigin Research Center, Fairylands Environmental Sci-Tech (Shenzhen) Co. Ltd., Shenzhen 518055, China.
出版信息
Bioresour Technol. 2023 Nov;388:129753. doi: 10.1016/j.biortech.2023.129753. Epub 2023 Sep 9.
The quorum quenching (QQ) strategy has attracted increasing attention in membrane bioreactor (MBR) fouling control. However, the applicable QQ strain remains limited. This study investigated the antibiofouling performance of a new indigenous QQ bacterium, Delftia sp. JL5 (JL5) in MBR. JL5 produces intracellular acylase that irreversibly degrades N-acylhomoserine lactones (AHL), inhibited biofilm formation of quorum-sensing bacteria from activated sludge. During 120 days of operation, immobilized JL5 substantially delayed MBR biofouling by 2.1 and 2.9 times, at a flux rate of 30 L/(m·h) and 20 L/(m·h), respectively. A slower flux rate was favorable for effective mitigation of JL5 biofouling. JL5 reduced the AHL and extracellular polymeric substances of biocake without affecting the efficiency of waste removal. The presence of JL5 significantly changed the microbial structure of the membrane biocake, but not the activated sludge. Collectively, high activity, durability, and acid tolerance credited JL5 as a promising strain for QQ-MBR.
群体淬灭（QQ）策略在膜生物反应器（MBR）的污垢控制方面受到了越来越多的关注。然而，适用的QQ菌株仍然有限。本研究调查了一种新的本地QQ细菌代尔夫特菌属JL5（JL5）在MBR中的抗生物污垢性能。JL5产生细胞内酰基转移酶，可不可逆地降解N-酰基高丝氨酸内酯（AHL），抑制活性污泥中群体感应细菌的生物膜形成。在120天的运行过程中，固定化的JL5分别在通量为30 L/(m·h)和20 L/(m·h)时，将MBR生物污垢显著延迟了2.1倍和2.9倍。较低的通量速率有利于有效减轻JL5生物污垢。JL5降低了生物滤饼中的AHL和胞外聚合物，而不影响废物去除效率。JL5的存在显著改变了膜生物滤饼的微生物结构，但未改变活性污泥的微生物结构。总体而言，高活性、耐久性和耐酸性使JL5成为QQ-MBR中有前景的菌株。
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