Unit of Immunology and Chronic Disease, Institute of Environmental Medicine, Karolinska Institutet, Nobels Väg 13, 17165, Stockholm, Sweden.
Division of Cardiovascular Epidemiology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
Sci Rep. 2023 Sep 11;13(1):15011. doi: 10.1038/s41598-023-42264-1.
Malondialdehyde (MDA) is generated in oxidized LDL. It forms covalent protein adducts, and is recognized by antibodies (anti-MDA). We previously studied IgM anti-MDA, and here we focus on IgG, IgG1 and IgG2 anti-MDA in predicting cardiovascular disease (CVD). We determined, by ELISA, anti-MDA in a 7-year follow-up of 60-year-old men and women from Stockholm County (2039 men, 2193 women). We identified 210 incident CVD cases (defined as new events of myocardial infarction (MI), and hospitalization for angina pectoris) and ischemic stroke, and 620 age- and sex-matched controls. IgG anti-MDA was not associated with CVD. Median values only differed significantly for IgG1 anti-MDA among men, with lower levels among cases than controls (p = 0.039). High IgG1 anti-MDA (above 75th percentile) was inversely associated with CVD risk after adjustment for smoking, body mass index, type 2 diabetes, hyperlipidemia, and hypertension, (OR and 95% CI: 0.59; 0.40-0.89). After stratification by sex, this association emerged in men (OR and 95% CI: 0.46; 0.27-0.77), but not in women. IgG2 anti-MDA were associated with protection in the whole group and among men though weaker than IgG1 anti-MDA. IgG2 anti-MDA above the 75th percentile was associated with an increased risk of MI/angina in women (OR and 95% CI: 2.57; (1.08-6.16)). IgG1 and less so IgG2 anti-MDA are protection markers for CVD and MI/angina in the whole group and among men. However, IgG2 anti-MDA was a risk marker for MI/angina among women. These findings could have implications for both prediction and therapy.
丙二醛（MDA）在氧化的 LDL 中产生。它形成共价蛋白质加合物，并被抗体（抗 MDA）识别。我们之前研究了 IgM 抗 MDA，在这里我们专注于 IgG、IgG1 和 IgG2 抗 MDA 预测心血管疾病（CVD）。我们通过 ELISA 法测定了来自斯德哥尔摩县 60 岁男性和女性 7 年随访中的抗 MDA（2039 名男性，2193 名女性）。我们确定了 210 例新发 CVD 病例（定义为心肌梗死（MI）新发病例和心绞痛住院）和缺血性中风，以及 620 名年龄和性别匹配的对照。IgG 抗 MDA 与 CVD 无关。仅在男性中 IgG1 抗 MDA 的中位数存在显著差异，病例组的水平低于对照组（p=0.039）。校正吸烟、体重指数、2 型糖尿病、高脂血症和高血压后，高 IgG1 抗 MDA（高于 75 百分位数）与 CVD 风险呈负相关（OR 和 95%CI：0.59；0.40-0.89）。按性别分层后，这种关联出现在男性中（OR 和 95%CI：0.46；0.27-0.77），但在女性中没有。IgG2 抗 MDA 在整个组和男性中与保护相关，尽管不如 IgG1 抗 MDA 强。高于第 75 百分位数的 IgG2 抗 MDA 与女性 MI/心绞痛的风险增加相关（OR 和 95%CI：2.57；（1.08-6.16））。在整个组和男性中，IgG1 和较少的 IgG2 抗 MDA 是 CVD 和 MI/心绞痛的保护标志物。然而，IgG2 抗 MDA 是女性 MI/心绞痛的风险标志物。这些发现可能对预测和治疗都有影响。
