Li Kaihuai, Ma Chaoyun, Xiong Chunlan, Zhou Xue, Mao Yahui, Wang Yong, Liu Fengquan
Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang 550025, China.
Key Laboratory of Agricultural Microbiology, College of Agriculture, Guizhou University, Guiyang 550025, China.
Phytopathology. 2024 Mar;114(3):512-520. doi: 10.1094/PHYTO-07-23-0264-R. Epub 2024 Mar 18.
Diffusible signal factor (DSF) family signals represent a unique group of quorum sensing (QS) chemicals that modulate a wide range of behaviors for bacteria to adapt to different environments. However, whether DSF-mediated QS signaling acts as a public language to regulate the behavior of biocontrol and pathogenic bacteria remains unknown. In this study, we present groundbreaking evidence demonstrating that RpfF or RpfF could be a conserved DSF-family signal synthase in or . Interestingly, we found that both RpfF and RpfF have the ability to synthesize DSF and BDSF signaling molecules. DSF and BDSF positively regulate the biosynthesis of an antifungal factor (heat-stable antifungal factor, HSAF) in . Finally, we show that RpfF and RpfF have similar functions in regulating HSAF production in , as well as the virulence, synthesis of virulence factors, biofilm formation, and extracellular polysaccharide production in . These findings reveal a previously uncharacterized mechanism of DSF-mediated regulation in both biocontrol and pathogenic bacteria.
可扩散信号因子（DSF）家族信号代表了一类独特的群体感应（QS）化学物质，可调节细菌的多种行为，使其适应不同环境。然而，DSF介导的群体感应信号是否作为一种通用语言来调节生防菌和病原菌的行为仍不清楚。在本研究中，我们提供了突破性证据，证明RpfF或RpfF可能是[具体细菌1]或[具体细菌2]中保守的DSF家族信号合成酶。有趣的是，我们发现RpfF和RpfF都有能力合成DSF和BDSF信号分子。DSF和BDSF正向调节[具体细菌3]中抗真菌因子（热稳定抗真菌因子，HSAF）的生物合成。最后，我们表明RpfF和RpfF在调节[具体细菌3]中HSAF的产生以及[具体细菌4]的毒力、毒力因子合成、生物膜形成和胞外多糖产生方面具有相似的功能。这些发现揭示了DSF介导的生防菌和病原菌调控机制，此前尚未被描述。
