Department of Science and Technology, Faculty of Applied Sciences, Uva Wellassa University, Passara Road, Badulla, Sri Lanka.
Department of Science and Technology, Faculty of Applied Sciences,Uva Wellassa University, Passara Road, Badulla, Sri Lanka.
Mol Biol Rep. 2023 Nov;50(11):8901-8914. doi: 10.1007/s11033-023-08741-6. Epub 2023 Sep 12.
Synthetic dyes are one of the main pollutants in the textile industry and bioremediation is considered as an environmentally friendly method to degrade them. Soil microbial consortia (MCs) are reported having the potential of decolorizing most of textile dyes. This study aimed at evaluating dye-degrading ability of MCs developed from fungi and bacteria.
Fungi and bacteria were isolated from the soil samples obtained from textile waste dumping site at Horana industrial zone, Sri Lanka and were screened for crystal violet (CV) and congo red (CR) dyes to develop MCs. Decolorization assay was performed for MCs along with individual isolates under variable pH levels. Metabolized products were characterized to confirm the biodegradation. A. tamari (F5) and P. putida (B3) significantly (P < 0.05) decolorized both dyes. All the MCs showed higher decolorization percentages over the individual microorganisms. Neutral pH (pH 7) was the optimum pH for the decolorization of both dyes by individual isolates and the percentages were significantly changed under the acidic and basic pH levels. However, decolorization ability by all MCs was not significantly changed with pH. Consortium with A. tamari - P. putida significantly (P < 0.05) decolourized both dyes under optimum pH 7.
All MCs showed better pH tolerance in degrading CV and CR. Thus, it can be concluded that the selected MC with A. tamari - P. putida can degrade CV and CR textile dyes efficiently into non-toxic compounds against plants under neutral pH. Degradation and decolorization of textile azo dyes by effective fungal-bacterial consortium.
合成染料是纺织工业的主要污染物之一，生物修复被认为是一种降解它们的环保方法。土壤微生物群落（MCs）被报道具有使大多数纺织染料脱色的潜力。本研究旨在评估从真菌和细菌中开发的 MCs 的染料降解能力。
从斯里兰卡霍拉纳工业区的纺织废料倾倒场获得的土壤样本中分离出真菌和细菌，并对结晶紫（CV）和刚果红（CR）染料进行筛选，以开发 MCs。在不同 pH 值下，对 MCs 以及单个分离物进行脱色试验。对代谢产物进行了特征分析，以确认生物降解。A. tamari（F5）和 P. putida（B3）显著（P < 0.05）降解了两种染料。所有 MCs 对两种染料的脱色率均高于单个微生物。中性 pH（pH 7）是两种染料单独分离物脱色的最佳 pH，在酸性和碱性 pH 水平下，百分比显著变化。然而，所有 MCs 的脱色能力不受 pH 值影响。A. tamari-P. putida 组成的共生体在最佳 pH 7 下显著（P < 0.05）降解了两种染料。
所有 MCs 在降解 CV 和 CR 时表现出更好的 pH 耐受性。因此，可以得出结论，所选的 A. tamari-P. putida 有效真菌-细菌共生体可以在中性 pH 下有效地将 CV 和 CR 纺织染料降解为对植物无毒的化合物。
