College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.
College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.
J Food Prot. 2023 Dec;86(12):100158. doi: 10.1016/j.jfp.2023.100158. Epub 2023 Sep 11.
Fungal spoilage of food is a worldwide concern prompting the development of many antimicrobial agents and applications. In this study, the cell-free supernatant (CFS) of Lacticaseibacillus paracasei ALAC-4 had a significant inhibition effect on fungi. The CFS with antifungal activities were combined with chitosan (CS) matrix to prepare an active packaging CS-CFS films by using a solvent casting method and used for the packaging of Mongolian cheese for 15 days during storage at 4 ± 1℃. The optimized formulation of the film were 1.25% (w/v) chitosan, 1.75% (w/v) gelatin, 0.3% (v/v) glycerol, and 9.6% (w/v) CFS. It was found that CS-CFS films exhibited strong antifungal activities against molds and yeasts, especially Candida albicans, and also had excellent mechanical properties. Additionally, FTIR spectroscopy indicated that hydrogen bonds between the CFS and CS formed, and there was a smooth surface, compact cross-section observed in SEM morphologies of CS-CFS films. Furthermore, CS-CFS film also displayed a strong antifungal effect against molds and yeasts on cheese surface. These results suggest that the chitosan-based CS-CFS film has a promising application for Mongolian cheese and food preservation.
真菌对食物的破坏是一个全球性的问题，促使人们开发了许多抗菌剂和应用。在这项研究中，副干酪乳杆菌 ALAC-4 的无细胞上清液（CFS）对真菌有显著的抑制作用。具有抗真菌活性的 CFS 与壳聚糖（CS）基质结合，通过溶剂浇铸法制备活性包装 CS-CFS 薄膜，并用于在 4±1℃下储存的蒙古奶酪包装 15 天。该薄膜的最佳配方为 1.25%（w/v）壳聚糖、1.75%（w/v）明胶、0.3%（v/v）甘油和 9.6%（w/v）CFS。结果表明，CS-CFS 薄膜对霉菌和酵母菌，特别是白色念珠菌具有很强的抗真菌活性，同时具有良好的机械性能。此外，傅里叶变换红外光谱（FTIR）表明 CFS 和 CS 之间形成了氢键，并且在 CS-CFS 薄膜的 SEM 形貌中观察到表面光滑、横截面致密。此外，CS-CFS 薄膜对奶酪表面的霉菌和酵母菌也具有很强的抗真菌作用。这些结果表明，基于壳聚糖的 CS-CFS 薄膜在蒙古奶酪和食品保鲜方面具有广阔的应用前景。
