基于CRISPR/Cas9的烟草基因靶向突变体的高通量创建
High-throughput creation of Nicotiana tabacum gene-targeted mutants based on CRISPR/Cas9.
作者信息
Chen Yudong, Xiang Haiying, Jia Ling, Yang Qianxu, Zhang Jianduo, Jiang Jiarui, Zeng Wanli, Deng Lele, Jin Jingjing, Gao Qian, Li Xuemei
机构信息
Yunnan Academy of Tobacco Science, Kunming, 650106, China.
Biological Science Research Center, Southwest University, Chongqing, 400715, China.
出版信息
Plant Cell Rep. 2023 Dec;42(12):2039-2042. doi: 10.1007/s00299-023-03050-5. Epub 2023 Sep 12.
PMID:37700173
Abstract
4382 available sgRNAs targeting 1060 tobacco genes were obtained, and 10,682 targeted mutants were created using high-throughput methods. Four optimization experiments were established to solve problems encountered during genetic transformation.
摘要
获得了4382个靶向1060个烟草基因的可用sgRNA，并使用高通量方法创建了10682个靶向突变体。建立了四个优化实验来解决基因转化过程中遇到的问题。
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