Zhao Zhuo, Bai Jing, Liu Chang, Wang Yansong, Wang Shuang, Zhao Furong, Gu Qiufang
Intensive Care Unit, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, 121001, China.
School of Pharmaceutical Sciences, Liaoning University, Shenyang, 110036, China.
J Clin Biochem Nutr. 2023 Sep;73(2):161-171. doi: 10.3164/jcbn.22-110. Epub 2023 Sep 1.
Metabolic differences between colorectal cancer (CRC) and NI (NI) play an important role in early diagnoses and in-time treatments. We investigated the metabolic alterations between CRC patients and NI, and identified some potential biomarkers, and these biomarkers might be used as indicators for diagnosis of CRC. In this study, there were 79 NI, 50 CRC I patients, 52 CRC II patients, 56 CRC III patients, and 52 CRC IV patients. MS-MS was used to measure the metabolic alterations. Univariate and multivariate data analysis and metabolic pathway analysis were applied to analyze metabolic data and determine differential metabolites. These indicators revealed that amino acid and fatty acids could separate these groups. Several metabolites indicated an excellent variables capability in the separation of CRC patients and NI. Ornithine, arginine, octadecanoyl carnitine, palmitoyl carnitine, adipoyl carnitine, and butyryl carnitine/propanoyl carnitine were selected to distinguish the CRC patients and NI. And methionine and propanoyl carnitine, were directly linked to different stages of CRC. Receiver operating characteristics curves and variables importance in projection both represented an excellent performance of these metabolites. In conclusion, we assessed the difference between CRC patients and NI, which supports guidelines for an early diagnosis and effective treatment.
结直肠癌（CRC）与正常个体（NI）之间的代谢差异在早期诊断和及时治疗中起着重要作用。我们研究了CRC患者与NI之间的代谢改变，识别出一些潜在生物标志物，这些生物标志物可能用作CRC诊断指标。本研究中，有79名正常个体、50名I期CRC患者、52名II期CRC患者、56名III期CRC患者和52名IV期CRC患者。采用串联质谱（MS-MS）测量代谢改变。应用单变量和多变量数据分析以及代谢途径分析来分析代谢数据并确定差异代谢物。这些指标显示氨基酸和脂肪酸能够区分这些组。几种代谢物在区分CRC患者和正常个体方面显示出优异的变量能力。选择鸟氨酸、精氨酸、十八烷酰肉碱、棕榈酰肉碱、己二酰肉碱和丁酰肉碱/丙酰肉碱来区分CRC患者和正常个体。并且蛋氨酸和丙酰肉碱与CRC的不同阶段直接相关。受试者工作特征曲线和投影变量重要性均表明这些代谢物具有优异的性能。总之，我们评估了CRC患者与正常个体之间的差异，这为早期诊断和有效治疗提供了依据。
