L. Jacq. 和 L. 的乙醇提取物对从皮肤损伤中分离的葡萄球菌的抗菌作用。
Antibacterial Effect of Ethanolic Extracts of L. Jacq. and L. against Staphylococci Isolated from Skin Lesions.
机构信息
Department of Chemistry, Laboratory of Analytical Chemistry, Universidad Autónoma de Nuevo León, Faculty of Biological Sciences, San Nicolás de los Garza, Nuevo León C.P. 66455, Mexico.
Food Department, Laboratory of Functional Foods, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León C.P. 66455, Mexico.
出版信息
Biomed Res Int. 2023 Sep 4;2023:5584412. doi: 10.1155/2023/5584412. eCollection 2023.
BACKGROUND
The resistance to antibiotics shown by some dermatological pathogenic microorganisms has increased the interest of pharmaceutical and cosmetic industries in developing natural products that possess different biological activities, including antimicrobial effects.
METHODS
In the present investigation, the antibacterial activity of ethanolic extracts of aerial part and leaves and seed was evaluated against resistant strains of isolated from skin lesions and against ATCC 25923 (reference strain). Column chromatography (CC) and preparative thin-layer chromatography (PTLC) were used to obtain separate fractions of the seed extract of . We also determined the antimicrobial resistance of the strains against antibiotics using the agar disc diffusion assay. In addition, phytochemical screening was performed by colorimetric standard techniques.
RESULTS
seed extract showed the highest antibacterial activity with MBC from 2.3 g/mL to 19.5 g/mL without differences with gentamicin ( = 0.998). The isolated strain I showed the highest antimicrobial resistance against the tested antibiotics. PTLC-fractions of seed extract showed MBC from 3.2 g/mL to 40.7 g/mL against I and 25923 (reference), respectively, which suggests a synergistic effect of the secondary metabolites present in the crude ethanolic extract compared to its active PTLC-fractions, where only coumarins and compounds with lactone groups were detected in the phytochemical screening.
CONCLUSION
seed extract has promising effects that should be considered in further studies as an alternative or adjuvant in treating skin infections caused by staphylococci.
背景
一些皮肤科致病微生物对抗生素的耐药性增加了制药和化妆品行业开发具有不同生物活性的天然产品的兴趣，包括抗菌作用。
方法
在本研究中，评估了来自皮肤病变的分离株和 ATCC 25923（参考株）对 叶和地上部分以及种子的乙醇提取物的抗菌活性。采用柱层析（CC）和制备性薄层色谱（PTLC）对 种子提取物进行分离。我们还使用琼脂扩散法测定了菌株对抗生素的抗菌耐药性。此外，通过比色标准技术进行了植物化学筛选。
结果
种子提取物显示出最高的抗菌活性，MBC 为 2.3-19.5 g/ml，与庆大霉素无差异（=0.998）。分离株 I 对测试的抗生素表现出最高的抗菌耐药性。PTLC 种子提取物的馏分对 I 和 25923（参考）的 MBC 分别为 3.2-40.7 g/ml，这表明与粗乙醇提取物的活性 PTLC 馏分相比，次生代谢物存在协同作用，在植物化学筛选中仅检测到香豆素和内酯类化合物。
结论
种子提取物具有潜在的作用，应在进一步的研究中作为治疗葡萄球菌引起的皮肤感染的替代或辅助药物进行考虑。
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