Baker R E, Corner M A, Lammertse T, Furth E
Exp Neurol. 1986 Nov;94(2):426-30. doi: 10.1016/0014-4886(86)90115-9.
Organotypic explants of fetal mouse spinal cord-dorsal root ganglia were grown for 3 weeks in the presence of 10 mM magnesium ion, which effectively eliminated all recordable bioelectric activity throughout the culturing period. When tested in minimal essential medium, the chronically silenced explants had significantly fewer points from which spontaneous neuronal activity could be recorded. In addition, fewer points could be found that showed dorsal root ganglion-evoked responses, resulting from a greater tendency for the spinal cord activity to be restricted to the vicinity of the dorsally entering DRG fibers. These findings, therefore, support the hypothesis that spontaneous bioelectric activity is required for functional as well as structural maturation of neural networks.
在含有10 mM镁离子的条件下，将胎鼠脊髓-背根神经节的器官型外植体培养3周，在此培养期间，镁离子有效地消除了所有可记录的生物电活动。在最低限度基本培养基中进行测试时，长期沉默的外植体上可记录到自发神经元活动的点明显减少。此外，由于脊髓活动更倾向于局限于背侧进入背根神经节纤维的附近区域，因此显示背根神经节诱发反应的点也较少。因此，这些发现支持了这样一种假说，即自发生物电活动对于神经网络的功能以及结构成熟是必需的。
