Ramirez Jorge L, Valdivia Paola, Rosas-Puchuri Ulises, Valdivia Nereida L
Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Peru.
Department of Biology, The University of Oklahoma, Norman, Oklahoma, USA.
Mol Ecol Resour. 2023 Nov;23(8):1959-1965. doi: 10.1111/1755-0998.13864. Epub 2023 Sep 13.
An accurate species delimitation is critical for biological studies. In this context, the use of molecular techniques along with species delimitation methods would help to a rapid and accurate biodiversity assessment. The species delimitation methods cluster data sets of orthologous sequences in molecular operational taxonomic units (MOTU). In particular, the methods based on a single gene are easily integrated with the widely used DNA barcoding approach. We developed SPdel a user-friendly pipeline to integrate different single-gene species delimitation methods. SPdel is designed to calculate and compare MOTUs obtained by different species delimitation approaches. SPdel also outputs diverse ready-to-publish quality figures, that facilitate the interpretation of results. SPdel aims to help researchers use species delimitation methods that would improve biodiversity studies.
准确的物种界定对于生物学研究至关重要。在此背景下，使用分子技术以及物种界定方法将有助于快速准确地评估生物多样性。物种界定方法将直系同源序列的数据集聚类为分子操作分类单元（MOTU）。特别是，基于单个基因的方法很容易与广泛使用的DNA条形码方法相结合。我们开发了SPdel，这是一个用户友好的管道，用于整合不同的单基因物种界定方法。SPdel旨在计算和比较通过不同物种界定方法获得的MOTU。SPdel还输出各种可直接发表的高质量图表，便于结果的解释。SPdel旨在帮助研究人员使用能够改进生物多样性研究的物种界定方法。
