Service de Bactériologie-Virologie, CHRU de Tours, Université de Tours, 37044, Tours, France.
Assistance Publique - Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre, Hôpital Cochin, Service de Bactériologie, Université Paris Cité, 75014, Paris, France.
Eur J Clin Microbiol Infect Dis. 2023 Oct;42(10):1245-1250. doi: 10.1007/s10096-023-04656-z. Epub 2023 Sep 13.
Streptococcus agalactiae remains a major pathogen in human health, especially in neonatal infection. Detection in pregnant women is essential to initiate intrapartum antibiotic prophylaxis. This study compared the HiberGene loop-mediated isothermal amplification (LAMP) assay to culture, the reference method, for the detection of group B Streptococcus (GBS) in pregnant women.
This was a prospective multicenter study conducted in four French hospitals. Three hundred fifty-four non-redundant routine care vaginal swabs were analyzed by both methods, LAMP assay and culture. Clinicians and patients were blinded to the results of the LAMP assay.
Three hundred thirty-seven samples presented concordant results, 15 presented discordant results, and 2 were invalid using the LAMP assay (excluded from the study). Compared to culture, the LAMP assay had a sensitivity of 87.7%, a specificity of 98%, a negative predictive value of 97.6%, and a positive predictive value of 89.3%.
The HiberGene GBS LAMP assay is an easy test that possesses good performances compared with the reference method, culture. It could be used in case of emergency when a quick result is needed.
无乳链球菌仍然是人类健康的主要病原体，特别是在新生儿感染中。在孕妇中检测到它对于开始产时抗生素预防至关重要。本研究比较了 Hib erGene 环介导等温扩增（LAMP）检测法与培养法（参考方法），用于检测孕妇中的 B 群链球菌（GBS）。
这是一项在法国四家医院进行的前瞻性多中心研究。通过两种方法，即 LAMP 检测法和培养法，对 354 个非冗余的常规护理阴道拭子进行了分析。临床医生和患者对 LAMP 检测法的结果均不知情。
337 个样本的结果一致，15 个样本的结果不一致，2 个样本的 LAMP 检测法无效（排除在研究之外）。与培养法相比，LAMP 检测法的灵敏度为 87.7%，特异性为 98%，阴性预测值为 97.6%，阳性预测值为 89.3%。
Hib erGene GBS LAMP 检测法是一种简单的检测方法，与参考方法（培养法）相比具有良好的性能。当需要快速结果时，它可以在紧急情况下使用。
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