Abe H, Komiya T, Obinata T
Dev Biol. 1986 Nov;118(1):42-51. doi: 10.1016/0012-1606(86)90071-0.
The types of troponin-T (TNT) expressed in neonatal chicken breast muscle were examined by two-dimensional gel electrophoresis (2-D PAGE), immunoblotting, and peptide mapping. When troponin from neonatal chicken breast muscle or whole lysate of the muscle was displayed on 2-D PAGE, multiple spots were observed in the TNT region on the gel. They differed slightly from those in adult breast- and leg-type TNT, but were positively stained with the antibody specific for TN-T. These results indicate that multiple spots observed in the TNT region are all TNT isoforms. The TNT isoforms in the neonatal breast muscle were classified into two groups, based on size. Each group contained about five variants. The first group with a larger size was in the molecular weight range of adult breast TNT, while the smaller-sized second group was in the molecular weight range of adult leg TNT. Overall peptide map patterns of variants in the first group and also that of adult breast TNT resembled each other, whereas those of variants in the second group were similar to that of adult leg TNT. The TNT of adult breast-type appeared at about 2- to 3-weeks posthatch, and thereafter became a major TNT isoform.
通过二维凝胶电泳（2-D PAGE）、免疫印迹和肽图谱分析，研究了新生鸡胸肌中表达的肌钙蛋白-T（TNT）类型。当将新生鸡胸肌的肌钙蛋白或肌肉全裂解物在二维凝胶电泳上展示时，在凝胶上的TNT区域观察到多个斑点。它们与成年胸型和腿型TNT中的斑点略有不同，但用针对TN-T的特异性抗体呈阳性染色。这些结果表明，在TNT区域观察到的多个斑点均为TNT同工型。根据大小，将新生胸肌中的TNT同工型分为两组。每组包含约五个变体。第一组较大，分子量范围与成年胸型TNT相同，而较小的第二组分子量范围与成年腿型TNT相同。第一组变体的总体肽图谱模式与成年胸型TNT的相似，而第二组变体的则与成年腿型TNT的相似。成年胸型TNT在孵化后约2至3周出现，此后成为主要的TNT同工型。
