Cyr D G, Eales J G
Gen Comp Endocrinol. 1986 Jul;63(1):86-92. doi: 10.1016/0016-6480(86)90185-1.
Immature rainbow trout held at 12 +/- 1 degree were injected intraperitoneally with a fine saline suspension of sodium ipodate (5 mg/100 g body wt) every 3 days. Plasma 3,5,3'-triiodo-L-thyronine (T3) fell to 40% of control levels by Day 1 and remained at about this level for the duration of the study (22 days). Plasma L-thyroxine (T4) level was not altered on Day 1 but was lowered to 50% of control values by Days 7 and 22. Immersion of trout in T4 (2 micrograms/100 ml water) elevated plasma T4 but did not alter the ipodate suppression of plasma T3. Injection of control or ipodate-treated trout with [125I]T4, [125I]T3, or Na131I indicated that in addition to blocking T45'-monodeiodination to T3, ipodate also decreased plasma clearance of T4 and T3 and their removal by the bile. Ipodate did not alter the hepatosomatic index but did depress the hematocrit by 22 days, possibly due to the hypothyroid state. In conclusion, ipodate at a dose of 5 mg/100 g, approximately one-tenth of a lethal dose, is an effective acute and chronic hypothyroid agent to administer to trout.
将未成熟的虹鳟鱼饲养在12±1摄氏度的环境中，每隔3天腹腔注射一次碘番酸钠的精细生理盐水悬浮液（5毫克/100克体重）。到第1天时，血浆3,5,3'-三碘-L-甲状腺原氨酸（T3）降至对照水平的40%，并在研究期间（22天）一直维持在该水平左右。血浆L-甲状腺素（T4）水平在第1天时未发生变化，但在第7天和第22天时降至对照值的50%。将虹鳟鱼浸入T4（2微克/100毫升水）中可提高血浆T4水平，但不会改变碘番酸钠对血浆T3的抑制作用。给对照或经碘番酸钠处理的虹鳟鱼注射[125I]T4、[125I]T3或Na131I表明，碘番酸钠除了阻断T4向T3的5'-单碘化作用外，还降低了血浆中T4和T3的清除率及其经胆汁的清除。碘番酸钠不会改变肝体指数，但在22天时确实会使血细胞比容降低，这可能是由于甲状腺功能减退状态所致。总之，剂量为5毫克/100克（约为致死剂量的十分之一）的碘番酸钠是一种对虹鳟鱼有效的急性和慢性甲状腺功能减退剂。
