Su Fei, Xue Yin, Ye Shiyi, Yu Bin, Li Junxing, Xu Lihua, Yuan Xiufang
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310002, China.
Zhejiang Center of Animal Disease Control, Hangzhou, Zhejiang 310020, China.
Vaccine. 2023 Oct 6;41(42):6379-6390. doi: 10.1016/j.vaccine.2023.09.017. Epub 2023 Sep 12.
Porcine epidemic diarrhea virus (PEDV) is a main cause of severe enteric disease in piglets, leading to millions of dollars lost annually in the global pig industry. Parenteral vaccination is limited in generating sufficient mucosal immunity, which is crucial for early defense against PEDV. Here, we orally administered ginseng stem-leaf saponins (GSLS) to mice before parenteral vaccination and found that GSLS significantly enhanced the phagocytosis of dendritic cells, promoted the activities of CD4 T cells and increased PEDV-specific IgA antibodies in the intestinal mucosa. Transcriptomic results showed that the altered genes following GSLS treatment were mostly related to the immune response and metabolism. In addition, integrated analysis of the transcriptome and metabolome revealed that the mechanism by which GSLS enhances mucosal immunity may be associated with progesterone-related pathways. Further studies are needed to explore the detailed molecular mechanisms.
猪流行性腹泻病毒（PEDV）是仔猪严重肠道疾病的主要病因，每年给全球养猪业造成数百万美元的损失。肠胃外疫苗接种在产生足够的粘膜免疫方面存在局限性，而粘膜免疫对于早期抵御PEDV至关重要。在此，我们在肠胃外疫苗接种前给小鼠口服人参茎叶皂苷（GSLS），发现GSLS显著增强了树突状细胞的吞噬作用，促进了CD4 T细胞的活性，并增加了肠道粘膜中PEDV特异性IgA抗体。转录组学结果表明，GSLS处理后改变的基因大多与免疫反应和代谢有关。此外，转录组和代谢组的综合分析表明，GSLS增强粘膜免疫的机制可能与孕酮相关途径有关。需要进一步研究以探索详细的分子机制。
