Poletaev A I, Makarov V L, Sveshnikov P G, Kondrat'eva N O, Vol'kenshteĭn M V
Mol Biol (Mosk). 1977 Jul-Aug;11(4):917-32.
Anisotropy of torsional strength of the splitted electronic transition in the case of chromophore-chromophore interaction of dye molecules situated on the helical matrix was considered theoretically and as analytical expression for the value Rperpendicular/Rparallel was obtained. These theoretical results were compared with the experimental data obtained with DNA-proflavine, DNA-pyronine and DNA-acridine orange complexes oriented in multicappilar flow-cell. Studies of the optical effects (optical density and CD changes) due to orientation of these complexes showed that the acridine chromophores are not perpendicular with respect to the DNA axis (alpha D = 19--22 degrees). The DNA base pairs in complexes as assumed also are not perpendicular to the DNA axis, the inclination angle of their transition moments (for the band near 260 nm) being bigger than that of dye chromophores (24 degrees). These results indicate that under experimental conditions used by us no intercalation can be observed.
理论上考虑了位于螺旋基质上的染料分子发色团 - 发色团相互作用情况下分裂电子跃迁扭转强度的各向异性，并得到了(R_{垂直}/R_{平行})值的解析表达式。将这些理论结果与在多毛细管流动池中取向的DNA - 原黄素、DNA - 派洛宁和DNA - 吖啶橙复合物获得的实验数据进行了比较。对这些复合物取向引起的光学效应（光密度和圆二色性变化）的研究表明，吖啶发色团相对于DNA轴不垂直（(\alpha_D = 19 - 22)度）。复合物中的DNA碱基对如所假设的那样也不垂直于DNA轴，其跃迁矩（对于260nm附近的波段）的倾斜角大于染料发色团的倾斜角（24度）。这些结果表明，在我们使用的实验条件下，未观察到插入现象。
